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Scientific article
English

Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Published inJournal of bacteriology, vol. 193, no. 9, p. 2332-2335
Publication date2011
Abstract

Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

Keywords
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Gene Expression Regulation, Bacterial/physiology
  • Genetic Fitness
  • Genome, Bacterial
  • Mutation
  • Staphylococcus aureus/classification/genetics/pathogenicity
  • Stress, Physiological
  • Virulence
  • Virulence Factors/genetics/metabolism
Citation (ISO format)
NAIR, Dhanalakshmi et al. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. In: Journal of bacteriology, 2011, vol. 193, n° 9, p. 2332–2335. doi: 10.1128/JB.00027-11
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Article (Published version)
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ISSN of the journal0021-9193
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