Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David; Bard, Jonathan; Beaume, Marie; Gill, Steven; Francois, Patrice; Cheung, Ambrose L

doi:10.1128/JB.00027-11

Scientific article

English

Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

ContributorsNair, Dhanalakshmi; Memmi, Guido; Hernandez, David; Bard, Jonathan; Beaume, Marie; Gill, Steven; Francois, Patrice

; Cheung, Ambrose L

Published inJournal of bacteriology, vol. 193, no. 9, p. 2332-2335

Publication date2011

Abstract

Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

Keywords

Chromosome Mapping
Chromosomes, Bacterial
Gene Expression Regulation, Bacterial/physiology
Genetic Fitness
Genome, Bacterial
Mutation
Staphylococcus aureus/classification/genetics/pathogenicity
Stress, Physiological
Virulence
Virulence Factors/genetics/metabolism

Affiliation

Faculté de médecine / Section de médecine clinique / Département de médecine

Research group

Analyse génomique et fonctionnelle du staphylocoque doré (604)

Citation (ISO format)

NAIR, Dhanalakshmi et al. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. In: Journal of bacteriology, 2011, vol. 193, n° 9, p. 2332–2335. doi: 10.1128/JB.00027-11

Article (Published version)

Identifiers

PID : unige:25385
DOI : 10.1128/JB.00027-11
PMID : 21378186

ISSN of the journal0021-9193

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Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

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