Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David; Bard, Jonathan; Beaume, Marie; Gill, Steven; Francois, Patrice; Cheung, Ambrose L

doi:10.1128/JB.00027-11

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Title		Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain
Authors		Nair, Dhanalakshmi Memmi, Guido Hernandez, David Bard, Jonathan Beaume, Marie Gill, Steven Francois, Patrice Cheung, Ambrose L
Published in		Journal of bacteriology. 2011, vol. 193, no. 9, p. 2332-5
Abstract		Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.
Keywords		Chromosome Mapping — Chromosomes, Bacterial — Gene Expression Regulation, Bacterial/physiology — Genetic Fitness — Genome, Bacterial — Mutation — Staphylococcus aureus/classification/genetics/pathogenicity — Stress, Physiological — Virulence — Virulence Factors/genetics/metabolism
Identifiers		DOI: 10.1128/JB.00027-11 PMID: 21378186
Full text		Article (Published version) (1.1 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research group		Analyse génomique et fonctionnelle du staphylocoque doré (604)
Citation (ISO format)		NAIR, Dhanalakshmi et al. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. In: Journal of bacteriology, 2011, vol. 193, n° 9, p. 2332-5. doi: 10.1128/JB.00027-11 https://archive-ouverte.unige.ch/unige:25385