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Title

Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics
Authors
Chicha, Laurie
Feki, Anis
Boni, Alessandro
Irion, Olivier
Hovatta, Outi
Jaconi, Marisa
Published in PLOS ONE. 2011, vol. 6, no. 2, p. e14733
Abstract Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In particular, the use of a fully defined medium (FDM) would avoid biases induced by unidentified factors contained in serum, and would also allow key molecular mediators involved in such a process to be identified. Our goal was to induce in vitro, the differentiation of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) into morphologically and phenotypically mature leukocytes and erythrocytes, in the complete absence of serum and feeder cells.
Keywords Animals — Antigens, CD34/metabolism — Cell Culture Techniques — Cell Differentiation/drug effects — Cell Lineage/physiology — Cell Size — Cells, Cultured — Culture Media/chemistry/pharmacology — Culture Media, Serum-Free/pharmacology — Embryonic Stem Cells/metabolism/physiology — Hematopoietic Stem Cells/cytology/metabolism/physiology — Humans — Induced Pluripotent Stem Cells/cytology/drug effects/physiology — Myeloid Cells/cytology/metabolism/physiology — Pluripotent Stem Cells/drug effects/physiology
Identifiers
PMID: 21364915
Full text
Article (Published version) (1.6 MB) - public document Free access
Structures
Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research groups Martinez De Tejada Begona (272)
Mécanismes de différentiation cellulaire des cellules cardiaques (536)
Citation
(ISO format) 		CHICHA, Laurie et al. Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics. In: PLOS ONE, 2011, vol. 6, n° 2, p. e14733. doi: 10.1371/journal.pone.0014733 https://archive-ouverte.unige.ch/unige:25380

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Deposited on : 2013-01-11

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