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Scientific article
Open access
English

Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics

Published inPloS one, vol. 6, no. 2, e14733
Publication date2011
Abstract

Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In particular, the use of a fully defined medium (FDM) would avoid biases induced by unidentified factors contained in serum, and would also allow key molecular mediators involved in such a process to be identified. Our goal was to induce in vitro, the differentiation of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) into morphologically and phenotypically mature leukocytes and erythrocytes, in the complete absence of serum and feeder cells.

Keywords
  • Animals
  • Antigens, CD34/metabolism
  • Cell Culture Techniques
  • Cell Differentiation/drug effects
  • Cell Lineage/physiology
  • Cell Size
  • Cells, Cultured
  • Culture Media/chemistry/pharmacology
  • Culture Media, Serum-Free/pharmacology
  • Embryonic Stem Cells/metabolism/physiology
  • Hematopoietic Stem Cells/cytology/metabolism/physiology
  • Humans
  • Induced Pluripotent Stem Cells/cytology/drug effects/physiology
  • Myeloid Cells/cytology/metabolism/physiology
  • Pluripotent Stem Cells/drug effects/physiology
Citation (ISO format)
CHICHA, Laurie et al. Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics. In: PloS one, 2011, vol. 6, n° 2, p. e14733. doi: 10.1371/journal.pone.0014733
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal1932-6203
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342downloads

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