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Title

Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics

Authors
Hovatta, Outi
Published in PLOS ONE. 2011, vol. 6, no. 2, p. e14733
Abstract Differentiation of pluripotent stem cells in vitro provides a powerful means to investigate early developmental fates, including hematopoiesis. In particular, the use of a fully defined medium (FDM) would avoid biases induced by unidentified factors contained in serum, and would also allow key molecular mediators involved in such a process to be identified. Our goal was to induce in vitro, the differentiation of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) into morphologically and phenotypically mature leukocytes and erythrocytes, in the complete absence of serum and feeder cells.
Keywords AnimalsAntigens, CD34/metabolismCell Culture TechniquesCell Differentiation/drug effectsCell Lineage/physiologyCell SizeCells, CulturedCulture Media/chemistry/pharmacologyCulture Media, Serum-Free/pharmacologyEmbryonic Stem Cells/metabolism/physiologyHematopoietic Stem Cells/cytology/metabolism/physiologyHumansInduced Pluripotent Stem Cells/cytology/drug effects/physiologyMyeloid Cells/cytology/metabolism/physiologyPluripotent Stem Cells/drug effects/physiology
Identifiers
PMID: 21364915
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Article (Published version) (1.6 MB) - public document Free access
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Research groups Groupe Irion Olivier/Boulvain Michel (gynécologie et obstétrique) (272)
Mécanismes de différentiation cellulaire des cellules cardiaques (536)
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CHICHA-GAUDIMIER, Laurie et al. Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics. In: PLOS ONE, 2011, vol. 6, n° 2, p. e14733. https://archive-ouverte.unige.ch/unige:25380

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Deposited on : 2013-01-11

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