Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

Rondas, Dieter; Tomas, Alejandra; Halban, Philippe A.

doi:10.2337/db10-0946

Article scientifique

Accès libre

Anglais

Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

Contributeurs/tricesRondas, Dieter; Tomas, Alejandra; Halban, Philippe A.

Publié dansDiabetes, vol. 60, no. 4, p. 1146-1157

Date de publication2011

Résumé

Actin cytoskeleton remodeling is known to be involved in glucose-stimulated insulin secretion (GSIS). We have observed glucose-stimulated changes at the β-cell basal membrane similar to focal adhesion remodeling in cell migration. This led us to study the role of two key focal adhesion proteins, focal adhesion kinase (FAK) and paxillin, in GSIS.

Mots-clés

Animals
Blotting, Western
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Flavonoids/pharmacology
Fluorescent Antibody Technique
Focal Adhesion Protein-Tyrosine Kinases/metabolism
Focal Adhesions/drug effects/metabolism
Glucose/pharmacology
Insulin/metabolism
Microscopy, Confocal
Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism
Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/metabolism
Paxillin/metabolism
Phosphorylation/drug effects
RNA Interference
Rats

Structure d'affiliation

Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

Groupe de recherche

Fonction de l'îlot de Langerhans (324)

Financement

Swiss National Science Foundation - Role of actin cytoskeleton remodelling in pancreatic beta cell function and survival [31003A_127276]

Citation (format ISO)

RONDAS, Dieter, TOMAS, Alejandra, HALBAN, Philippe A. Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin. In: Diabetes, 2011, vol. 60, n° 4, p. 1146–1157. doi: 10.2337/db10-0946

Article (Accepted version)

Identifiants

PID : unige:25378
DOI : 10.2337/db10-0946
PMID : 21357465

ISSN du journal0012-1797

552vues

279téléchargements

Création16/10/2012 10:09:00

Première validation16/10/2012 10:09:00

Heure de mise à jour14/03/2023 19:56:06

Changement de statut14/03/2023 19:56:06

Dernière indexation16/01/2024 00:53:14

Archive ouverte UNIGE

Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

Informations techniques