Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

Rondas, Dieter; Tomas, Alejandra; Halban, Philippe A.

doi:10.2337/db10-0946

Scientific article

Open access

English

Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

ContributorsRondas, Dieter; Tomas, Alejandra; Halban, Philippe A.

Published inDiabetes, vol. 60, no. 4, p. 1146-1157

Publication date2011

Abstract

Actin cytoskeleton remodeling is known to be involved in glucose-stimulated insulin secretion (GSIS). We have observed glucose-stimulated changes at the β-cell basal membrane similar to focal adhesion remodeling in cell migration. This led us to study the role of two key focal adhesion proteins, focal adhesion kinase (FAK) and paxillin, in GSIS.

Keywords

Animals
Blotting, Western
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Flavonoids/pharmacology
Fluorescent Antibody Technique
Focal Adhesion Protein-Tyrosine Kinases/metabolism
Focal Adhesions/drug effects/metabolism
Glucose/pharmacology
Insulin/metabolism
Microscopy, Confocal
Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism
Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/metabolism
Paxillin/metabolism
Phosphorylation/drug effects
RNA Interference
Rats

Affiliation

Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

Research group

Fonction de l'îlot de Langerhans (324)

Funding

Swiss National Science Foundation - Role of actin cytoskeleton remodelling in pancreatic beta cell function and survival [31003A_127276]

Citation (ISO format)

RONDAS, Dieter, TOMAS, Alejandra, HALBAN, Philippe A. Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin. In: Diabetes, 2011, vol. 60, n° 4, p. 1146–1157. doi: 10.2337/db10-0946

Article (Accepted version)

Identifiers

PID : unige:25378
DOI : 10.2337/db10-0946
PMID : 21357465

ISSN of the journal0012-1797

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Focal adhesion remodeling is crucial for glucose-stimulated insulin secretion and involves activation of focal adhesion kinase and paxillin

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