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Chapitre de livre
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Self-Assembly of PNA-Encoded Peptides into Microarrays

Publié dansPeptide Microarrays: Methods and Protocols, Editeurs/trices Cretich, M. & Chiari, M., p. 299-307
Maison d'éditionHumana Press
Collection
  • Methods in Molecular Biology; 570
Date de publication2009
Résumé

Several technologies have been described to immobilize libraries of small molecules or peptides in a microarray format. Herein, we describe protocols for an alternative strategy whereby each small molecule or peptide within a library is labeled with a peptide nucleic acid (PNA) tag such that they self-assemble in a microarray format upon hybridization with readily available DNA arrays. An important asset of the method is that it allows the library to be used in solution prior to hybridizing and as such offers the opportunity to separate the inhibitors bound to the protein from the rest of the library. Two methods based on size exclusion filtration or gel electrophoresis to separate protein-bound inhibitors from the remaining library are described.

Structure d'affiliation Pas une publication de l'UNIGE
Citation (format ISO)
DEBAENE, Francois, WINSSINGER, Nicolas. Self-Assembly of PNA-Encoded Peptides into Microarrays. In: Peptide Microarrays: Methods and Protocols. [s.l.] : Humana Press, 2009. p. 299–307. (Methods in Molecular Biology) doi: 10.1007/978-1-60327-394-7_15
Identifiants
ISBN978-1-60327-393-0
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Informations techniques

Création12/12/2012 09:30:00
Première validation12/12/2012 09:30:00
Heure de mise à jour14/03/2023 17:48:37
Changement de statut14/03/2023 17:48:37
Dernière indexation16/01/2024 00:43:14
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