Scientific Article
previous document  unige:24738  next document
add to browser collection
Title

PNA-Encoded Protease Substrate Microarrays

Authors
Damoiseaux, Robert
Tully, David C.
Geierstanger, Bernhard H.
Burdick, Keith
Harris, Jennifer L.
Published in Chemistry & Biology. 2004, vol. 11, no. 10, p. 1351-1360
Abstract Our current understanding of the role and regulation of protease activity in normal and pathogenic processes is limited by our ability to measure and deconvolute their enzymatic activity. To address this limitation, an approach was developed that utilizes rhodamine-based fluorogenic substrates encoded with PNA tags. The PNA tags address each of the substrates to a predefined location on an oligonucleotide microarray through hybridization, thus allowing the deconvolution of multiple signals from a solution. A library of 192 protease substrates was prepared by split and mix combinatorial synthesis. The methodology and validation of this approach for profiling proteolytic activity from single proteases and from those in crude cell lysates as well as clinical blood samples is described.
Identifiers
Full text
Citation
(ISO format)
WINSSINGER, Nicolas et al. PNA-Encoded Protease Substrate Microarrays. In: Chemistry & Biology, 2004, vol. 11, n° 10, p. 1351-1360. https://archive-ouverte.unige.ch/unige:24738

165 hits

1 download

Update

Deposited on : 2012-12-19

Export document
Format :
Citation style :