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Title

Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Authors
Pianowski, Zbigniew
Gorska, Katarzyna
Oswald, Laurence
Merten, Christoph A.
Published in Journal of the American Chemical Society. 2009, vol. 131, no. 18, p. 6492-6497
Abstract Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.
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PIANOWSKI, Zbigniew et al. Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes. In: Journal of the American Chemical Society, 2009, vol. 131, n° 18, p. 6492-6497. doi: 10.1021/ja809656k https://archive-ouverte.unige.ch/unige:24717

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Deposited on : 2012-12-18

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