Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Pianowski, Zbigniew; Gorska, Katarzyna; Oswald, Laurence; Merten, Christoph A.; Winssinger, Nicolas

doi:10.1021/ja809656k

Scientific article

English

Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Contributors

Pianowski, Zbigniew; Gorska, Katarzyna; Oswald, Laurence; Merten, Christoph A.; Winssinger, Nicolas

Published in

Journal of the American Chemical Society, vol. 131, no. 18, p. 6492-6497

Publication date

2009

Abstract

Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

PIANOWSKI, Zbigniew et al. Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes. In: Journal of the American Chemical Society, 2009, vol. 131, n° 18, p. 6492–6497. doi: 10.1021/ja809656k

Article (Published version)

Identifiers

PID : unige:24717
DOI : 10.1021/ja809656k

Journal ISSN

0002-7863

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Creation

12/12/2012 09:39:00

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12/12/2012 09:39:00

Update

13/10/2025 23:52:39

Status update

14/03/2023 17:47:50

Last indexation

01/07/2026 06:09:56

Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation