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Scientific article
English

Profiling protein function with small molecule microarrays

Publication date2002
Abstract

The regulation of protein function through posttranslational modification, local environment, and protein–protein interaction is critical to cellular function. The ability to analyze on a genome-wide scale protein functional activity rather than changes in protein abundance or structure would provide important new insights into complex biological processes. Herein, we report the application of a spatially addressable small molecule microarray to an activity-based profile of proteases in crude cell lysates. The potential of this small molecule-based profiling technology is demonstrated by the detection of caspase activation upon induction of apoptosis, characterization of the activated caspase, and inhibition of the caspase-executed apoptotic phenotype using the small molecule inhibitor identified in the microarray-based profile.

Affiliation Not a UNIGE publication
Citation (ISO format)
WINSSINGER, Nicolas et al. Profiling protein function with small molecule microarrays. In: Proceedings of the National Academy of Sciences of the United States of America, 2002, vol. 99, n° 17, p. 11139–11144. doi: 10.1073/pnas.172286899
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ISSN of the journal0027-8424
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