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Scientific article
English

PNA Encoding (PNA=peptide nucleic acid): From Solution-Based Libraries to Organized Microarrays

Contributors

Harris, Jennifer L.; Winssinger, Nicolas

Published in

Chemistry, vol. 11, no. 23, p. 6792-6801

Publication date

2005

Abstract

Microarray-based technologies have attracted attention in chemical biology by virtue of their miniaturized format, which is well suited to probe ligand–protein interactions or investigate enzymatic activity in complex biological mixtures. A number of research groups have reported the preparation of surfaces on microarrays with specific functional groups to chemoselectively attach small molecules from libraries. We have developed an alternative method whereby libraries are encoded with peptide nucleic acid (PNA), such that libraries which exist as mixtures in solution self-assemble into an organized microarray through hybridization to produce readily available DNA arrays. This allows libraries synthesized by split and mix methods to be decoded in a single step. An asset of this method compared to direct spotting is that libraries can be used in solution for bioassays prior to self-assembly into the microarray format.

Keywords

  • combinatorial chemistry
  • microarrays
  • PNA
  • self-assembly

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

HARRIS, Jennifer L., WINSSINGER, Nicolas. PNA Encoding (PNA=peptide nucleic acid): From Solution-Based Libraries to Organized Microarrays. In: Chemistry, 2005, vol. 11, n° 23, p. 6792–6801. doi: 10.1002/chem.200500305

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0947-6539
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Technical informations

Creation

11/12/2012 17:04:00

First validation

11/12/2012 17:04:00

Update

13/10/2025 23:53:13

Status update

14/03/2023 17:47:13

Last indexation

01/07/2026 06:09:16
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