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Scientific article
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English

Preventing mitochondrial fission impairs mitochondrial function and leads to loss of mitochondrial DNA

Contributors

Parone, Philippe; Da Cruz, Sandrine; Tondera, Daniel; Mattenberger, Yves Benoît; James, Dominic I.; Maechler, Pierre; Barja, François; Martinou, Jean-Claude

Published in

PloS one, vol. 3, no. 9, e3257

Publication date

2008

Abstract

Mitochondria form a highly dynamic tubular network, the morphology of which is regulated by frequent fission and fusion events. However, the role of mitochondrial fission in homeostasis of the organelle is still unknown. Here we report that preventing mitochondrial fission, by down-regulating expression of Drp1 in mammalian cells leads to a loss of mitochondrial DNA and a decrease of mitochondrial respiration coupled to an increase in the levels of cellular reactive oxygen species (ROS). At the cellular level, mitochondrial dysfunction resulting from the lack of fission leads to a drop in the levels of cellular ATP, an inhibition of cell proliferation and an increase in autophagy. In conclusion, we propose that mitochondrial fission is required for preservation of mitochondrial function and thereby for maintenance of cellular homeostasis.

Keywords

  • Adenosine Triphosphate/metabolism
  • Autophagy
  • Cell Proliferation
  • DNA, Mitochondrial/metabolism
  • GTP Phosphohydrolases/metabolism
  • Gene Expression Regulation
  • Hela Cells
  • Homeostasis
  • Humans
  • Membrane Potential, Mitochondrial
  • Microtubule-Associated Proteins/metabolism
  • Mitochondria/metabolism/physiology
  • Mitochondrial Proteins/metabolism
  • Models, Biological
  • Oxygen Consumption
  • RNA Interference
  • Reactive Oxygen Species

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

PARONE, Philippe et al. Preventing mitochondrial fission impairs mitochondrial function and leads to loss of mitochondrial DNA. In: PloS one, 2008, vol. 3, n° 9, p. e3257. doi: 10.1371/journal.pone.0003257

Main files (1)

Article (Published version)
CC BY

Identifiers

Journal ISSN

1932-6203
753views
475downloads

Technical informations

Creation

13/08/2009 15:44:00

First validation

13/08/2009 15:44:00

Update

14/03/2023 15:10:32

Status update

14/03/2023 15:10:32

Last indexation

29/10/2024 11:53:47
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