UNIGE document Scientific Article
previous document  unige:24174  next document
add to browser collection
Title

Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication

Authors
Published in Molecular Cell. 2007, vol. 25, no. 3, p. 345-55
Abstract We investigated the dynamics of histone-DNA interactions in yeast by using inducible forms of epitope-tagged histones H2B and H3. Chromatin assembly of newly synthesized histones was assessed by chromatin immunoprecipitation in G1-arrested cells to prevent replication-coupled histone incorporation. We find that while histone deposition within a subtelomeric region is strictly linked to DNA replication, histone H2B is continuously incorporated at the promoter and coding regions of both transcriptionally active and inactive loci. In contrast, incorporation of histone H3 occurs only at active genes, being predominant at the promoter and showing a dynamics along the gene that inversely correlates with the average nucleosomal density. Similar results were obtained with N-terminally truncated H2B and H3 variants. We infer that replication-independent incorporation of H2B and H3 are distinct events, each occurring independently of the histone tail, and that nucleosome loss at active promoters reflects a dynamic equilibrium between histone deposition and dissociation.
Keywords Cell Cycle/physiologyChromatin Assembly and DisassemblyChromatin ImmunoprecipitationDNA ReplicationEpigenesis, GeneticHistones/chemistry/metabolismNucleosomes/metabolismPromoter Regions, GeneticSaccharomyces cerevisiae/geneticsSaccharomyces cerevisiae Proteins/geneticsTranscription, Genetic
Identifiers
PMID: 17289583
Full text
Article (Published version) (1.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Article (Published version) (239 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Régulation de l'expression des gènes du virus de l'hépatite B (294)
Project FNS: 31003A-127384
Citation
(ISO format)
JAMAI, Adil, IMOBERDORF, Rachel, STRUBIN, Michel. Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication. In: Molecular Cell, 2007, vol. 25, n° 3, p. 345-55. https://archive-ouverte.unige.ch/unige:24174

206 hits

2 downloads

Update

Deposited on : 2012-11-21

Export document
Format :
Citation style :