Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication

Article Article scientifique Article Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication eng 2007 author Jamai Adil 558464 author Imoberdorf Rachel 514345 author Strubin Michel 22429 345-355 10.1016/j.molcel.2007.01.019 17289583 1097-2765 We investigated the dynamics of histone-DNA interactions in yeast by using inducible forms of epitope-tagged histones H2B and H3. Chromatin assembly of newly synthesized histones was assessed by chromatin immunoprecipitation in G1-arrested cells to prevent replication-coupled histone incorporation. We find that while histone deposition within a subtelomeric region is strictly linked to DNA replication, histone H2B is continuously incorporated at the promoter and coding regions of both transcriptionally active and inactive loci. In contrast, incorporation of histone H3 occurs only at active genes, being predominant at the promoter and showing a dynamics along the gene that inversely correlates with the average nucleosomal density. Similar results were obtained with N-terminally truncated H2B and H3 variants. We infer that replication-independent incorporation of H2B and H3 are distinct events, each occurring independently of the histone tail, and that nucleosome loss at active promoters reflects a dynamic equilibrium between histone deposition and dissociation. Cell Cycle/physiology Chromatin Assembly and Disassembly Chromatin Immunoprecipitation DNA Replication Epigenesis, Genetic Histones/chemistry/metabolism Nucleosomes/metabolism Promoter Regions, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics Transcription, Genetic Dewey 616 Swiss National Science Foundation Part I: Dynamics of Histone Exchange and Epigenetic Modifications during Transcription in vivo Part II: Hijacking of the DDB1 Ubiquitin Ligase by the HBV X Protein: What Role during Infection and Associated Liver Cancer? 127384 CH Project funding 54 Département de microbiologie et médecine moléculaire 21B false 230 Régulation de l'expression des gènes du virus de l'hépatite B 294 Molecular cell 25 3 d557c051-566f-48d1-8716-9e1e9e718bf8 Article (Published version) Restricted Jamai et al., 2007.pdf 1683274 application/pdf 1e8020ca-70b1-4bc8-a813-ed01a9ebd1b2 Article (Published version) Restricted Jamai et al., 2007 Suppl.pdf 244559 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Continuous histone H2B and transcription-dependent histone H3 exchange in yeast cells outside of replication	Molecular cell			2007	708	0

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Imoberdorf, Rachel