en
Scientific article
English

Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein

Published inDevelopmental dynamics, vol. 240, no. 10, p. 2335-2343
Publication date2011
Abstract

In mammals, the Prostaglandin D(2) (PGD(2) ) signaling pathway is involved in male gonadal development, regulating Sox9 gene expression and SOX9 protein subcellular localization through lipocalin prostaglandin D synthase (L-Pgds) activity. Nevertheless, because L-Pgds is downstream of Sox9, its expression cannot explain the initial nuclear translocation of the SOX9 protein. Here, we show that another source of PGD(2) , hematopoietic-Pgds (H-Pgds) enzyme is expressed in somatic and germ cells of the embryonic gonad of both sexes, as early as embryonic day (E) 10.5, before the onset of L-Pgds expression. Inhibition of H-Pgds activity by the specific HQL-79 inhibitor leads to impaired nuclear translocation of SOX9 protein in E11.5 Sertoli cells. Furthermore, analysis of H-Pgds(-/-) male embryonic gonads confirms abnormal subcellular localization of SOX9 protein at the E11.5 early stage of mouse testicular differentiation suggesting a role for H-Pgds-produced PGD(2) in the initial nuclear translocation of SOX9.

Keywords
  • Active Transport, Cell Nucleus/physiology
  • Animals
  • Cell Nucleus/metabolism
  • Female
  • Gonads/cytology/embryology/metabolism
  • Humans
  • Isomerases/genetics/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Prostaglandin D2/metabolism
  • SOX9 Transcription Factor/genetics/metabolism
  • Sertoli Cells/cytology/metabolism
Funding
  • Swiss National Science Foundation - fnrs
Citation (ISO format)
MONIOT, Brigitte et al. Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein. In: Developmental dynamics, 2011, vol. 240, n° 10, p. 2335–2343. doi: 10.1002/dvdy.22726
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Article (Published version)
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Identifiers
ISSN of the journal1058-8388
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