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Title

Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein

Authors
Moniot, Brigitte
Farhat, Andalib
Aritake, Kosuke
Declosmenil, Faustine
Eguchi, Naomi
Urade, Yoshihiro
Poulat, Francis
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Published in Developmental Dynamics. 2011, vol. 240, no. 10, p. 2335-43
Abstract In mammals, the Prostaglandin D(2) (PGD(2) ) signaling pathway is involved in male gonadal development, regulating Sox9 gene expression and SOX9 protein subcellular localization through lipocalin prostaglandin D synthase (L-Pgds) activity. Nevertheless, because L-Pgds is downstream of Sox9, its expression cannot explain the initial nuclear translocation of the SOX9 protein. Here, we show that another source of PGD(2) , hematopoietic-Pgds (H-Pgds) enzyme is expressed in somatic and germ cells of the embryonic gonad of both sexes, as early as embryonic day (E) 10.5, before the onset of L-Pgds expression. Inhibition of H-Pgds activity by the specific HQL-79 inhibitor leads to impaired nuclear translocation of SOX9 protein in E11.5 Sertoli cells. Furthermore, analysis of H-Pgds(-/-) male embryonic gonads confirms abnormal subcellular localization of SOX9 protein at the E11.5 early stage of mouse testicular differentiation suggesting a role for H-Pgds-produced PGD(2) in the initial nuclear translocation of SOX9.
Keywords Active Transport, Cell Nucleus/physiologyAnimalsCell Nucleus/metabolismFemaleGonads/cytology/embryology/metabolismHumansIsomerases/genetics/metabolismMaleMiceMice, Inbred C57BLMice, KnockoutProstaglandin D2/metabolismSOX9 Transcription Factor/genetics/metabolismSertoli Cells/cytology/metabolism
Identifiers
PMID: 21887724
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Article (Published version) (5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Mécanismes moléculaires et génétiques du développement sexuel (778)
Project FNS: fnrs
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(ISO format)
MONIOT, Brigitte et al. Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein. In: Developmental Dynamics, 2011, vol. 240, n° 10, p. 2335-43. https://archive-ouverte.unige.ch/unige:23482

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Deposited on : 2012-10-18

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