Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein

Moniot, Brigitte; Farhat, Andalib; Aritake, Kosuke; Declosmenil, Faustine; Nef, Serge; Eguchi, Naomi; Urade, Yoshihiro; Poulat, Francis; Boizet-Bonhoure, Brigitte

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Title		Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein
Authors		Moniot, Brigitte Farhat, Andalib Aritake, Kosuke Declosmenil, Faustine Nef, Serge Eguchi, Naomi Urade, Yoshihiro Poulat, Francis Boizet-Bonhoure, Brigitte show all authors [1 - 9]
Published in		Developmental Dynamics. 2011, vol. 240, no. 10, p. 2335-43
Abstract		In mammals, the Prostaglandin D(2) (PGD(2) ) signaling pathway is involved in male gonadal development, regulating Sox9 gene expression and SOX9 protein subcellular localization through lipocalin prostaglandin D synthase (L-Pgds) activity. Nevertheless, because L-Pgds is downstream of Sox9, its expression cannot explain the initial nuclear translocation of the SOX9 protein. Here, we show that another source of PGD(2) , hematopoietic-Pgds (H-Pgds) enzyme is expressed in somatic and germ cells of the embryonic gonad of both sexes, as early as embryonic day (E) 10.5, before the onset of L-Pgds expression. Inhibition of H-Pgds activity by the specific HQL-79 inhibitor leads to impaired nuclear translocation of SOX9 protein in E11.5 Sertoli cells. Furthermore, analysis of H-Pgds(-/-) male embryonic gonads confirms abnormal subcellular localization of SOX9 protein at the E11.5 early stage of mouse testicular differentiation suggesting a role for H-Pgds-produced PGD(2) in the initial nuclear translocation of SOX9.
Keywords		Active Transport, Cell Nucleus/physiology — Animals — Cell Nucleus/metabolism — Female — Gonads/cytology/embryology/metabolism — Humans — Isomerases/genetics/metabolism — Male — Mice — Mice, Inbred C57BL — Mice, Knockout — Prostaglandin D2/metabolism — SOX9 Transcription Factor/genetics/metabolism — Sertoli Cells/cytology/metabolism
Identifiers		DOI: 10.1002/dvdy.22726 PMID: 21887724
Full text		Article (Published version) (5 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Research group		Mécanismes moléculaires et génétiques du développement sexuel (778)
Project		FNS: fnrs
Citation (ISO format)		MONIOT, Brigitte et al. Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein. In: Developmental Dynamics, 2011, vol. 240, n° 10, p. 2335-43. https://archive-ouverte.unige.ch/unige:23482