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Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes

Publié dansBiology of reproduction, vol. 85, no. 5, p. 1025-1039
Date de publication2011
Résumé

In mammals, female meiosis consists of two asymmetric cell divisions, which generate a large haploid oocyte and two small polar bodies. Asymmetric partitioning of the cytoplasm results from migration of the meiotic spindle toward the cortex and requires actin filaments. However, the subcellular localization and the role of the existing two cytoplasmic actin (CYA) isoforms, beta and gamma, have not been characterized. We show that beta- and gamma-CYA are differentially distributed in the maturing oocyte from late metaphase I as well as in preimplantation embryos. Gamma-CYA is preferentially enriched in oocyte cortices and is absent from all cell-cell contact areas from metaphase II until the blastocyst stage. Beta-CYA is enriched in contractile structures, at cytokinesis, at cell-cell contacts, and around the forming blastocoel. Alteration of beta- or gamma-CYA function by isoform-specific antibody microinjection suggests that gamma-CYA holds a major and specific role in the establishment and/or maintenance of asymmetry in meiosis I and in the maintenance of overall cortical integrity. In contrast, beta- and gamma-CYA, together, appear to participate in the formation and the cortical anchorage of the second meiotic spindle in waiting for fertilization. Finally, differences in gamma-CYA expression are amongst the earliest markers of cell fate determination in development.

Mots-clés
  • Actins/genetics/immunology/physiology
  • Animals
  • Antibodies/administration & dosage/immunology/pharmacology
  • Cell Communication/physiology
  • Cell Differentiation/physiology
  • Cell Polarity
  • Cells, Cultured
  • Cytoplasm/physiology
  • Female
  • Meiosis/drug effects/physiology
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Microinjections
  • Models, Animal
  • Oocytes/cytology/physiology
Financement
  • Swiss National Science Foundation - FNRS
Citation (format ISO)
BROCKMANN, Céline Denise et al. Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes. In: Biology of reproduction, 2011, vol. 85, n° 5, p. 1025–1039. doi: 10.1095/biolreprod.111.091736
Fichiers principaux (1)
Article (Published version)
accessLevelPublic
Identifiants
ISSN du journal0006-3363
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1056téléchargements

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Création10/10/2012 16:01:00
Première validation10/10/2012 16:01:00
Heure de mise à jour14/03/2023 17:43:11
Changement de statut14/03/2023 17:43:11
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