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Doctoral thesis
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Role of STIM (Stromal interaction molecule) in Store operated calcium entry

ContributorsShen, Weiwei
Defense date2012-06-26
Abstract

Store operated calcium entry (SOCE) is controlled by the ER-resident Stromal Interaction Molecule 1 (STIM1) which senses a decrease in [Ca2+]ER and opens Ca2+-selective channels of the Orai and TRPC families. The oligomerization of STIM1 initiated by a drop in [Ca2+]ER is the crucial step for SOCE activation, and by analogy SOCE termination is thought to be controlled by the de-oligomerization of STIM1 initiated by the restoration of physiological [Ca2+]ER levels. We quantify the [Ca2+]ER dependence of STIM1 oligo-/deoligo-merization during physiological stimulation of HeLa cells, using the D1ER to measure [Ca2+]ER and FRET between CFP-STIM1 and YFP-STIM1 to monitor STIM1 oligo-/deoligo-merization. We found that the kinetics of STIM1 oligomerization follow the kinetics of store depletion independently of the type of stimulation used. We also showed that, in addition to store refilling, a local increase in the cytosolic Ca2+ concentration is required to initiate STIM1 de-oligomerization.

eng
Keywords
  • Calcium
  • STIM
  • SOCE
Citation (ISO format)
SHEN, Weiwei. Role of STIM (Stromal interaction molecule) in Store operated calcium entry. 2012. doi: 10.13097/archive-ouverte/unige:23015
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Creation09/16/2012 3:01:00 PM
First validation09/16/2012 3:01:00 PM
Update time03/14/2023 5:41:13 PM
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