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Title

Macrophage migration inhibitory factor deficiency leads to age-dependent impairment of glucose homeostasis in mice

Authors
Serre-Beinier, Veronique
Calandra, Thierry
Roger, Thierry
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Published in Journal of Endocrinology. 2010, vol. 206, no. 3, p. 297-306
Abstract Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine produced by many cells and tissues including pancreatic beta-cells, liver, skeletal muscle, and adipocytes. This study investigates the potential role of MIF in carbohydrate homeostasis in a physiological setting outside of severe inflammation, utilizing Mif knockout (MIF-/-) mice. Compared with wild-type (WT) mice, MIF-/- mice had a lower body weight, from birth until 4 months of age, but subsequently gained weight faster, resulting in a higher body weight at 12 months of age. The lower weight in young mice was related to a higher energy expenditure, and the higher weight in older mice was related to an increased food intake and a higher fat mass. Fasting blood insulin level was higher in MIF-/- mice compared with WT mice at any age. After i.p. glucose injection, the elevation of blood insulin level was higher in MIF-/- mice compared with WT mice, at 2 months of age, but was lower in 12-month-old MIF-/- mice. As a result, the glucose clearance during intraperitoneal glucose tolerance tests was higher in MIF-/- mice compared with WT mice until 4 months of age, and was lower in 12-month-old MIF-/- mice. Insulin resistance was estimated (euglycemic-hyperinsulinemic clamp tests), and the phosphorylation activity of AKT was similar in MIF-/- mice and WT mice. In conclusion, this mouse model provides evidence for the role of MIF in the control of glucose homeostasis.
Keywords Age FactorsAnalysis of VarianceAnimalsBlood Glucose/ metabolismBlotting, WesternBody Composition/physiologyBody Weight/physiologyEnergy Metabolism/physiologyGlucose Clamp TechniqueGlucose Tolerance TestHomeostasis/ physiologyInsulin/metabolismInsulin Resistance/physiologyLeptin/bloodLipids/bloodMacrophage Migration-Inhibitory Factors/genetics/ metabolismMiceMice, Inbred C57BLMice, KnockoutMotor Activity/physiologyReverse Transcriptase Polymerase Chain ReactionStatistics, Nonparametric
Identifiers
PMID: 20566490
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Structures
Research groups Chirurgie viscérale (104)
Imagerie et radiologie interventionnelle du foie, des voies biliaires et du pancréas (316)
Cultures cellulaires et transplantations (519)
Lipoprotéines sériques (598)
La transplantation d'îlots de Langerhans (623)
Homéostasie métabolique (649)
Transplantation et hépatologie (905)
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SERRE-BEINIER, Veronique et al. Macrophage migration inhibitory factor deficiency leads to age-dependent impairment of glucose homeostasis in mice. In: Journal of Endocrinology, 2010, vol. 206, n° 3, p. 297-306. https://archive-ouverte.unige.ch/unige:21308

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Deposited on : 2012-05-23

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