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Title

Sclerocarya birrea (Anacardiaceae) stem-bark extract corrects glycaemia in diabetic rats and acts on beta-cells by enhancing glucose-stimulated insulin secretion

Authors
Makom Ndifossap, Ivonne Gisele
Ngueguim Tsofack, Florence
Dongo, Etienne
Kamtchouing, Pierre
Dimo, Theophile
Published in Journal of Endocrinology. 2010, vol. 205, no. 1, p. 79-86
Abstract Sclerocarya birrea is a plant widely used as traditional medication for the treatment of diabetes in sub-Saharan regions. However, the mechanism of action is unknown and only hypoglycaemic effects of S. birrea extract (SBE) in diabetic rats have been reported to date. Here, we tested aqueous extracts of S. birrea on insulin-secreting INS-1E cells and isolated rat islets. Following 24 h of treatment at 5 microg/ml, the extract markedly potentiated glucose-stimulated insulin secretion. Neither basal insulin release nor non-nutrient stimulation was affected. The potentiation of the secretory response at stimulatory glucose appeared after 12 h of treatment. No acute effects were observed and, at the effective concentration, SBE was safe regarding cell integrity and differentiation. The mechanism of action of the SBE was related to glucose metabolism as both ATP generation and glucose oxidation were enhanced following the 24-h treatment. In streptozotocin-induced diabetic rats, SBE administration corrected glycaemia and restored plasma insulin levels after 2 weeks of treatment. These data show direct action of S. birrea on insulin-secreting cells and favour further delineation for use of the plant in the management of diabetes.
Keywords Adenosine Triphosphate/metabolism*AnacardiaceaeAnimalsCell Line, TumorDiabetes Mellitus, Experimental/chemically induced/*drug therapy/metabolismDisease Models, AnimalGlucose/metabolism/*pharmacologyHyperglycemia/*drug therapy/metabolismInsulin/*metabolismInsulin-Secreting Cells/drug effects/*metabolismInsulinoma/metabolism/pathologyMalePancreatic Neoplasms/metabolism/pathologyPlant BarkPlant Extracts/pharmacology/*therapeutic usePlant StemsRatsRats, WistarStreptozocin
Identifiers
PMID: 20061513
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Research group Mitochondries et sécrétion d'insuline (671)
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(ISO format)
MAKOM NDIFOSSAP, Ivonne Gisele et al. Sclerocarya birrea (Anacardiaceae) stem-bark extract corrects glycaemia in diabetic rats and acts on beta-cells by enhancing glucose-stimulated insulin secretion. In: Journal of Endocrinology, 2010, vol. 205, n° 1, p. 79-86. https://archive-ouverte.unige.ch/unige:21105

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Deposited on : 2012-05-23

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