Marker-independent identification of glioma-initiating cells

Clement, Virginie; Marino, Denis; Cudalbu, Cristina; Hamou, Marie-France; Mlynarik, Vladimir; De Tribolet, Nicolas; Dietrich, Pierre-Yves; Gruetter, Rolf; Hegi, M. E.; Radovanovic, Ivan

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Title		Marker-independent identification of glioma-initiating cells
Authors		Clement, Virginie Marino, Denis Cudalbu, Cristina Hamou, Marie-France Mlynarik, Vladimir De Tribolet, Nicolas Dietrich, Pierre-Yves Gruetter, Rolf Hegi, M. E. Radovanovic, Ivan show all authors [1 - 10]
Published in		Nature Methods. 2010, vol. 7, no. 3, p. 224-228
Abstract		Tumor-initiating cells with stem cell properties are believed to sustain the growth of gliomas, but proposed markers such as CD133 cannot be used to identify these cells with sufficient specificity. We report an alternative isolation method purely based on phenotypic qualities of glioma-initiating cells (GICs), avoiding the use of molecular markers. We exploited intrinsic autofluorescence properties and a distinctive morphology to isolate a subpopulation of cells (FL1(+)) from human glioma or glioma cultures. FL1(+) cells are capable of self-renewal in vitro, tumorigenesis in vivo and preferentially express stem cell genes. The FL1(+) phenotype did not correlate with the expression of proposed GIC markers. Our data propose an alternative approach to investigate tumor-initiating potential in gliomas and to advance the development of new therapies and diagnostics.
Keywords		Animals — Antigens, CD/analysis — Brain Neoplasms/pathology — Cell Differentiation — Cells, Cultured — Fluorescence — Gene Expression Profiling — Glioma/pathology — Glycoproteins/analysis — Humans — Mice — Neoplastic Stem Cells/pathology — Peptides/analysis — Tumor Markers, Biological/analysis
Identifiers		DOI: 10.1038/nmeth.1430 PMID: 20173750
Full text		Article - Limited access to UNIGE Other version: http://www.nature.com/nmeth/journal/v7/n3/pdf/nmeth.1430.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques
Research group		Immunothérapie des cancers (42)
Citation (ISO format)		CLEMENT, Virginie et al. Marker-independent identification of glioma-initiating cells. In: Nature Methods, 2010, vol. 7, n° 3, p. 224-228. https://archive-ouverte.unige.ch/unige:20852