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Scientific article
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Open access
English

Distinct clinical phenotypes associated with JAK2V617F reflect differential STAT1 signaling

Published inCancer cell, vol. 18, no. 5, p. 524-535
Publication date2010
Abstract

The JAK2V617F mutation is associated with distinct myeloproliferative neoplasms, including polycythemia vera (PV) and essential thrombocythemia (ET), but it remains unclear how it generates disparate disorders. By comparing clonally-derived mutant and wild-type cells from individual patients, we demonstrate that the transcriptional consequences of JAK2V617F are subtle, and that JAK2V617F-heterozygous erythroid cells from ET and PV patients exhibit differential interferon signaling and STAT1 phosphorylation. Increased STAT1 activity in normal CD34-positive progenitors produces an ET-like phenotype, whereas downregulation of STAT1 activity in JAK2V617F-heterozygous ET progenitors produces a PV-like phenotype. Our results illustrate the power of clonal analysis, indicate that the consequences of JAK2V617F reflect a balance between STAT5 and STAT1 activation and are relevant for other neoplasms associated with signaling pathway mutations.

Keywords
  • Amino Acid Substitution
  • Cell Culture Techniques
  • Cell Differentiation/genetics
  • Cells, Cultured
  • Cluster Analysis
  • Down-Regulation
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Heterozygote
  • Humans
  • Interferons/metabolism
  • Janus Kinase 2/*genetics
  • Megakaryocytes/pathology
  • Myeloproliferative Disorders/*genetics
  • Phenotype
  • Phosphorylation
  • Polycythemia Vera/*genetics
  • STAT1 Transcription Factor/genetics/*metabolism
  • STAT5 Transcription Factor/genetics/metabolism
  • Signal Transduction
  • Thrombocythemia, Essential/*genetics
  • Transcriptional Activation
Citation (ISO format)
CHEN, Edwin et al. Distinct clinical phenotypes associated with JAK2V617F reflect differential STAT1 signaling. In: Cancer cell, 2010, vol. 18, n° 5, p. 524–535. doi: 10.1016/j.ccr.2010.10.013
Main files (1)
Article (Accepted version)
accessLevelPublic
Identifiers
ISSN of the journal1535-6108
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642downloads

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