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Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

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Published in Journal of Medical Microbiology. 2009, vol. 58, no. Pt 1, p. 65-68
Abstract Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.
Keywords Arthritis, Infectious/*diagnosis/*microbiologyElbow Joint/microbiologyFemaleFoot Joints/microbiologyHumansKingella kingae/*isolation & purificationMaleNeisseriaceae Infections/*diagnosisPolymerase Chain Reaction/*methodsSensitivity and Specificity
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PMID: 19074654
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Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Groupe Lascombes Pierre (orthopédie pédiatrique) (917)
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CHERKAOUI, Abdessalam et al. Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay. In: Journal of Medical Microbiology, 2009, vol. 58, n° Pt 1, p. 65-68. https://archive-ouverte.unige.ch/unige:19739

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Deposited on : 2012-04-23

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