

Other version: http://jmm.sgmjournals.org/content/58/1/65.full.pdf#page=1&view=FitH
![]() |
Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay |
|
Authors | ||
Published in | Journal of Medical Microbiology. 2009, vol. 58, no. Pt 1, p. 65-68 | |
Abstract | Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens. | |
Keywords | Arthritis, Infectious/*diagnosis/*microbiology — Elbow Joint/microbiology — Female — Foot Joints/microbiology — Humans — Kingella kingae/*isolation & purification — Male — Neisseriaceae Infections/*diagnosis — Polymerase Chain Reaction/*methods — Sensitivity and Specificity | |
Identifiers | PMID: 19074654 | |
Full text |
![]() ![]() Other version: http://jmm.sgmjournals.org/content/58/1/65.full.pdf#page=1&view=FitH |
|
Structures | ||
Research groups | Analyse génomique et fonctionnelle du staphylocoque doré (604) Groupe Lascombes Pierre (orthopédie pédiatrique) (917) | |
Citation (ISO format) | CHERKAOUI, Abdessalam et al. Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay. In: Journal of Medical Microbiology, 2009, vol. 58, n° Pt 1, p. 65-68. doi: 10.1099/jmm.0.47707-0 https://archive-ouverte.unige.ch/unige:19739 |