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Title

Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay
Authors
Cherkaoui, Abdessalam
Ceroni, Dimitri
Emonet, Stéphane Paul
Lefevre, Yan
Schrenzel, Jacques
Published in Journal of Medical Microbiology. 2009, vol. 58, no. Pt 1, p. 65-68
Abstract Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.
Keywords Arthritis, Infectious/*diagnosis/*microbiology — Elbow Joint/microbiology — Female — Foot Joints/microbiology — Humans — Kingella kingae/*isolation & purification — Male — Neisseriaceae Infections/*diagnosis — Polymerase Chain Reaction/*methods — Sensitivity and Specificity
Identifiers
PMID: 19074654
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Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://jmm.sgmjournals.org/content/58/1/65.full.pdf#page=1&view=FitH
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Faculté de médecine / Section de médecine clinique / Département de pédiatrie
Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Groupe Lascombes Pierre (orthopédie pédiatrique) (917)
Citation
(ISO format) 		CHERKAOUI, Abdessalam et al. Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay. In: Journal of Medical Microbiology, 2009, vol. 58, n° Pt 1, p. 65-68. https://archive-ouverte.unige.ch/unige:19739

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Deposited on : 2012-04-23

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