Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

Cherkaoui, Abdessalam; Ceroni, Dimitri; Emonet, Stéphane; Lefevre, Yan; Schrenzel, Jacques

doi:10.1099/jmm.0.47707-0

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Title		Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay
Authors		Cherkaoui, Abdessalam Ceroni, Dimitri Emonet, Stéphane Lefevre, Yan Schrenzel, Jacques
Published in		Journal of Medical Microbiology. 2009, vol. 58, no. Pt 1, p. 65-68
Abstract		Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.
Keywords		Arthritis, Infectious/diagnosis/microbiology — Elbow Joint/microbiology — Female — Foot Joints/microbiology — Humans — Kingella kingae/isolation & purification — Male — Neisseriaceae Infections/diagnosis — Polymerase Chain Reaction/*methods — Sensitivity and Specificity
Identifiers		DOI: 10.1099/jmm.0.47707-0 PMID: 19074654
Full text		Article - Limited access to UNIGE Other version: http://jmm.sgmjournals.org/content/58/1/65.full.pdf#page=1&view=FitH
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research groups		Analyse génomique et fonctionnelle du staphylocoque doré (604) Groupe Lascombes Pierre (orthopédie pédiatrique) (917)
Citation (ISO format)		CHERKAOUI, Abdessalam et al. Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay. In: Journal of Medical Microbiology, 2009, vol. 58, n° Pt 1, p. 65-68. doi: 10.1099/jmm.0.47707-0 https://archive-ouverte.unige.ch/unige:19739