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Scientific article
Case report
English

Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

Published inJournal of Medical Microbiology, vol. 58, no. Pt 1, p. 65-68
Publication date2009
Abstract

Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.

Keywords
  • Arthritis, Infectious/*diagnosis/*microbiology
  • Elbow Joint/microbiology
  • Female
  • Foot Joints/microbiology
  • Humans
  • Kingella kingae/*isolation & purification
  • Male
  • Neisseriaceae Infections/*diagnosis
  • Polymerase Chain Reaction/*methods
  • Sensitivity and Specificity
Citation (ISO format)
CHERKAOUI, Abdessalam et al. Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay. In: Journal of Medical Microbiology, 2009, vol. 58, n° Pt 1, p. 65–68. doi: 10.1099/jmm.0.47707-0
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ISSN of the journal0022-2615
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