Societal gender role norms play a crucial role in shaping men's and women's career aspirations. However, prior research documenting this key role of gendered norms has primarily focused on heterosexual women and men in the global North‐West. Previous studies documenting differences in career interests by sexual orientation suggest that gender role norms might affect lesbian, gay and bisexual (LGB) women and men less strongly than heterosexuals. This large‐scale, preregistered study sought to document group differences in norm perceptions, career interests and their relationship. To this end, 18,351 university students ( n = 3318 LGB, n = 15,033 heterosexual) from 46 countries completed a self‐report survey about their gender, sexual orientation, perceptions of societal gender role norms, as well as their personal gender role beliefs and career interests. Analyses revealed that, compared to heterosexual individuals, LGB individuals reported significantly less stereotypical career interests and endorsed less traditional gender role beliefs about careers. As predicted, perceptions of societal gender role norms predicted career interests for heterosexual but not for LGB participants. Moreover, LGB participants showed a weaker relationship between societal gender role norms and own gender role beliefs than straight participants did. Interestingly, we found no evidence that this effect was conditional on the level of LGBT acceptance in different countries sampled. We discuss implications of this new evidence for understanding occupational gender segregation from an intersectional perspective.