Reconstitution of Rab9 endosomal targeting and nucleotide exchange using purified Rab9-GDP dissociation inhibitor complexes and endosome-enriched membranes
ContributorsSoldati, Thierry
Published inMethods in enzymology, vol. 257, p. 253-259
Publication date1995
Keywords
- Animals
- Cell Fractionation/methods
- Cell Line
- Centrifugation, Density Gradient/methods
- Cloning, Molecular/methods
- Electrophoresis, Polyacrylamide Gel/methods
- Endosomes/metabolism/ultrastructure
- GTP Phosphohydrolases/isolation & purification/metabolism
- GTP-Binding Proteins/isolation & purification/metabolism
- Guanine Nucleotide Dissociation Inhibitors
- Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
- Immunoblotting/methods
- Indicators and Reagents
- Liver/metabolism/ultrastructure
- Protein Prenylation
- Radioisotope Dilution Technique
- Rats
- Recombinant Proteins/isolation & purification/metabolism
- Spodoptera
- Sulfur Radioisotopes
- Transfection/methods
- Rab GTP-Binding Proteins
Affiliation Not a UNIGE publication
Citation (ISO format)
SOLDATI, Thierry, SHAPIRO, A D, PFEFFER, S R. Reconstitution of Rab9 endosomal targeting and nucleotide exchange using purified Rab9-GDP dissociation inhibitor complexes and endosome-enriched membranes. In: Methods in enzymology, 1995, vol. 257, p. 253–259. doi: 10.1016/s0076-6879(95)57030-6
Main files (1)
Article (Published version)
Identifiers
- PID : unige:19344
- DOI : 10.1016/s0076-6879(95)57030-6
- PMID : 8583928
ISSN of the journal0076-6879
