Scientific article
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English

Reconstitution of Rab9 endosomal targeting and nucleotide exchange using purified Rab9-GDP dissociation inhibitor complexes and endosome-enriched membranes

Published inMethods in enzymology, vol. 257, p. 253-259
Publication date1995
Keywords
  • Animals
  • Cell Fractionation/methods
  • Cell Line
  • Centrifugation, Density Gradient/methods
  • Cloning, Molecular/methods
  • Electrophoresis, Polyacrylamide Gel/methods
  • Endosomes/metabolism/ultrastructure
  • GTP Phosphohydrolases/isolation & purification/metabolism
  • GTP-Binding Proteins/isolation & purification/metabolism
  • Guanine Nucleotide Dissociation Inhibitors
  • Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
  • Immunoblotting/methods
  • Indicators and Reagents
  • Liver/metabolism/ultrastructure
  • Protein Prenylation
  • Radioisotope Dilution Technique
  • Rats
  • Recombinant Proteins/isolation & purification/metabolism
  • Spodoptera
  • Sulfur Radioisotopes
  • Transfection/methods
  • Rab GTP-Binding Proteins
Affiliation entities Not a UNIGE publication
Citation (ISO format)
SOLDATI, Thierry, SHAPIRO, A D, PFEFFER, S R. Reconstitution of Rab9 endosomal targeting and nucleotide exchange using purified Rab9-GDP dissociation inhibitor complexes and endosome-enriched membranes. In: Methods in enzymology, 1995, vol. 257, p. 253–259. doi: 10.1016/s0076-6879(95)57030-6
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
Journal ISSN0076-6879
511views
655downloads

Technical informations

Creation19/03/2012 16:36:00
First validation19/03/2012 16:36:00
Update time14/03/2023 18:22:06
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