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Wipe sampling procedure coupled to LC–MS/MS analysis for the simultaneous determination of 10 cytotoxic drugs on different surfaces

Contributors

Nussbaumer, Susanne; Geiser, Laurent; Sadeghipour, Farshid; Hochstrasser, Denis; Bonnabry, Pascal; Veuthey, Jean-Luc; Fleury-Souverain, Sandrine

Published in

Analytical & bioanalytical chemistry, vol. 402, no. 8, p. 2499-2509

Collection

  • Open Access - Licence nationale Springer

Publication date

2012

Abstract

A simple wipe sampling procedure was developed for the surface contamination determination of ten cytotoxic drugs: cytarabine, gemcitabine, methotrexate, etoposide phosphate, cyclophosphamide, ifosfamide, irinotecan, doxorubicin, epirubicin and vincristine. Wiping was performed using Whatman filter paper on different surfaces such as stainless steel, polypropylene, polystyrol, glass, latex gloves, computer mouse and coated paperboard. Wiping and desorption procedures were investigated: The same solution containing 20% acetonitrile and 0.1% formic acid in water gave the best results. After ultrasonic desorption and then centrifugation, samples were analysed by a validated liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) in selected reaction monitoring mode. The whole analytical strategy from wipe sampling to LC–MS/MS analysis was evaluated to determine quantitative performance. The lowest limit of quantification of 10 ng per wiping sample (i.e. 0.1 ng cm−2) was determined for the ten investigated cytotoxic drugs. Relative standard deviation for intermediate precision was always inferior to 20%. As recovery was dependent on the tested surface for each drug, a correction factor was determined and applied for real samples. The method was then successfully applied at the cytotoxic production unit of the Geneva University Hospitals pharmacy.

Keywords

  • Cytotoxic
  • Antineoplastic drugs
  • Surface contamination
  • Environmental monitoring
  • LC–MS/MS
  • Wipe sampling

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

NUSSBAUMER, Susanne et al. Wipe sampling procedure coupled to LC–MS/MS analysis for the simultaneous determination of 10 cytotoxic drugs on different surfaces. In: Analytical & bioanalytical chemistry, 2012, vol. 402, n° 8, p. 2499–2509. doi: 10.1007/s00216-011-5157-2

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

http://www.springerlink.com/index/10.1007/s00216-011-5157-2

Journal ISSN

1618-2642
844views
532downloads

Technical informations

Creation

27/03/2012 11:49:00

First validation

27/03/2012 11:49:00

Update

23/01/2025 11:45:20

Status update

23/01/2025 11:45:20

Last indexation

23/01/2025 11:50:47
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