Riverbed siltation in estuaries affects ecosystem functioning, water quality, and navigation. This study investigates the flow-regulated Osellino Canal, a freshwater tributary of the Venice Lagoon that crosses a largely urbanized area and is undergoing progressive siltation. High-resolution measurements of discharge (Q) and suspended sediment concentration (SSC) were performed using hydroacoustic instrumentation from September 2019 to December 2021. The analysis examined discharge dynamics, sediment transport, and rainfall-runoff relationships. Results indicate a mean annual discharge of 2.1 m3 s−1 and an average annual suspended sediment load of ~2900 ± 330 t. Discharge patterns were strongly influenced by water management, resulting in anomalous runoff coefficients (δ > 1) during dry periods. Sediment export proved to be strongly event-driven: episodic high-flow events accounted for about 23% of the total load despite representing only a small fraction of the study period. Furthermore, a strong linear relationship between runoff and sediment load (R2 = 0.94) confirms an advection-dominated regime, where net export is regulated primarily by hydrodynamic volume rather than fluctuations in sediment supply. Bathymetric comparisons (2011–2019) reveal a mean annual sediment retention of 400 ± 100 t yr-1, corresponding to a trapping efficiency of approximately 12 ± 3% relative to the gross sediment input. These findings, supported by SSL–runoff regression residuals, consistently indicate net sediment accumulation associated with the long-term malfunction of a miter-gate system that impedes efficient sediment export. This study provides a critical pre-rehabilitation baseline, establishing a benchmark to evaluate the effectiveness of ongoing restoration efforts initiated in March 2022 and the future hydromorphological recovery of the canal.