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Title

A unique chicken B-creatine kinase gene gives rise to two B-creatine kinase isoproteins with distinct N termini by alternative splicing

Authors
Wirz, T
Brändle, U
Hossle, J P
Perriard, J C
Published in Journal of Biological Chemistry. 1990, vol. 265, no. 20, p. 11656-66
Abstract In the chicken, a high degree of heterogeneity at the protein level has been reported for the creatine kinase-type B (B-CK). Here we show that the two B-CK isoproteins, Ba- and Bb-CK, are encoded by two mRNAs, which are derived from a single copy gene by a stochastic alternative splicing mechanism. The transcription of the single hnRNA is directed by a complex promoter region containing a stretch of sequences which is highly conserved among all the B-CK genes known to date. This stretch encompasses a putative binding site for the TA-rich DNA-binding protein (Hobson, G. M., Mitchell, M. T., Molloy, G. R., and Pearson, M. L. (1988) Nucleic Acids Res. 16, 8925-8944) which is located in the distal part of the promoter region, while the proximal portion containing the TATA-box used in vivo is not conserved between chicken and mammals. The two isoproteins arising from this gene contain distinct N-terminal portions. According to comparative analysis, Bb-CK is the form which is homologous to the mammalian B-CKs, whereas Ba-CK shows some sequence features unique among all other vertebrate cytosolic creatine kinases characterized so far.
Keywords Amino Acid SequenceAnimalsBase SequenceBlotting, SouthernChickensCloning, MolecularCreatine Kinase/geneticsDNA/blood/genetics/isolation & purificationDNA ProbesGene LibraryGenesIsoenzymesMolecular Sequence DataPolymerase Chain ReactionRNA SplicingRNA, Messenger/geneticsRestriction MappingSequence Homology, Nucleic AcidTranscription, Genetic
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PMID: 2365692
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WIRZ, T et al. A unique chicken B-creatine kinase gene gives rise to two B-creatine kinase isoproteins with distinct N termini by alternative splicing. In: Journal of Biological Chemistry, 1990, vol. 265, n° 20, p. 11656-66. https://archive-ouverte.unige.ch/unige:18973

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Deposited on : 2012-03-27

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