Scientific article
Open access

Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum

Published inMolecular biology of the cell, vol. 13, no. 4, p. 1390-1407
Publication date2002

Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study. D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.

  • Animals
  • Biological Transport
  • Cell Membrane/metabolism
  • Dictyostelium/physiology
  • Endocytosis
  • Endosomes/metabolism
  • Kinetics
  • Leukocytes/metabolism
  • Lysosomes/metabolism
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Time Factors
Affiliation Not a UNIGE publication
Citation (ISO format)
NEUHAUS, Eva M, ALMERS, Wolfhard, SOLDATI, Thierry. Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum. In: Molecular biology of the cell, 2002, vol. 13, n° 4, p. 1390–1407. doi: 10.1091/mbc.01-08-0392
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Article (Published version)
ISSN of the journal1059-1524

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