en
Scientific article
Open access
English

Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum

Published inMolecular biology of the cell, vol. 13, no. 4, p. 1390-1407
Publication date2002
Abstract

Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study. D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.

Keywords
  • Animals
  • Biological Transport
  • Cell Membrane/metabolism
  • Dictyostelium/physiology
  • Endocytosis
  • Endosomes/metabolism
  • Kinetics
  • Leukocytes/metabolism
  • Lysosomes/metabolism
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Time Factors
Affiliation Not a UNIGE publication
Citation (ISO format)
NEUHAUS, Eva M, ALMERS, Wolfhard, SOLDATI, Thierry. Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum. In: Molecular biology of the cell, 2002, vol. 13, n° 4, p. 1390–1407. doi: 10.1091/mbc.01-08-0392
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal1059-1524
467views
314downloads

Technical informations

Creation03/19/2012 12:38:00 PM
First validation03/19/2012 12:38:00 PM
Update time03/14/2023 5:10:06 PM
Status update03/14/2023 5:10:05 PM
Last indexation01/15/2024 11:07:47 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack