Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum

Neuhaus, Eva M; Almers, Wolfhard; Soldati, Thierry

doi:10.1091/mbc.01-08-0392

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Title		Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum
Authors		Neuhaus, Eva M Almers, Wolfhard Soldati, Thierry
Published in		Molecular biology of the cell. 2002, vol. 13, no. 4, p. 1390-407
Abstract		Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study. D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.
Keywords		Animals — Biological Transport — Cell Membrane/metabolism — Dictyostelium/physiology — Endocytosis — Endosomes/metabolism — Kinetics — Leukocytes/metabolism — Lysosomes/metabolism — Microscopy, Fluorescence — Microscopy, Video — Time Factors
Identifiers		DOI: 10.1091/mbc.01-08-0392 PMID: 11950947
Full text		Article (Published version) (1 MB) - Free access
Citation (ISO format)		NEUHAUS, Eva M, ALMERS, Wolfhard, SOLDATI, Thierry. Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum. In: Molecular biology of the cell, 2002, vol. 13, n° 4, p. 1390-407. doi: 10.1091/mbc.01-08-0392 https://archive-ouverte.unige.ch/unige:18918