Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts

Hagedorn, Monica; Rohde, Kyle H; Russell, David G; Soldati, Thierry

doi:10.1126/science.1169381

Scientific article

English

Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts

Contributors

Hagedorn, Monica; Rohde, Kyle H; Russell, David G; Soldati, Thierry

Published in

Science, vol. 323, no. 5922, p. 1729-1733

Publication date

2009

Abstract

To generate efficient vaccines and cures for Mycobacterium tuberculosis, we need a far better understanding of its modes of infection, persistence, and spreading. Host cell entry and the establishment of a replication niche are well understood, but little is known about how tubercular mycobacteria exit host cells and disseminate the infection. Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M. marinum, but not M. avium, are ejected from the cell through an actin-based structure, the ejectosome. This conserved nonlytic spreading mechanism requires a cytoskeleton regulator from the host and an intact mycobacterial ESX-1 secretion system. This insight offers new directions for research into the spreading of tubercular mycobacteria infections in mammalian cells.

Keywords

Actins/physiology
Animals
Bacterial Proteins/metabolism
Cell Membrane/microbiology
Cytoskeleton/microbiology/physiology/ultrastructure
Cytosol/microbiology
Dictyostelium/microbiology/ultrastructure
GTP Phosphohydrolases/metabolism
Mycobacterium avium/genetics/pathogenicity/physiology
Mycobacterium marinum/genetics/pathogenicity/physiology
Mycobacterium tuberculosis/genetics/pathogenicity/physiology
Phagocytosis
Pressure
Vacuoles/microbiology

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de biochimie

Citation (ISO format)

HAGEDORN, Monica et al. Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts. In: Science, 2009, vol. 323, n° 5922, p. 1729–1733. doi: 10.1126/science.1169381

Article (Published version)

Identifiers

PID : unige:18878
DOI : 10.1126/science.1169381
PMID : 19325115

Journal ISSN

0036-8075

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979downloads

Creation

19/03/2012 12:19:00

First validation

19/03/2012 12:19:00

Update

14/03/2023 17:09:55

Status update

14/03/2023 17:09:55

Last indexation

29/10/2024 19:07:15

Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation