Dynamin A, Myosin IB and Abp1 couple phagosome maturation to F-actin binding

Gopaldass, Navin Andréw; Patel, Devang; Kratzke, Ramona; Dieckmann, Régis; Hausherr, Stéphanie; Hagedorn, Monica; Monroy, Roger; Krüger, Julia; Neuhaus, Eva M; Hoffmann, Eik; Hille, Katja; Kuznetsov, Sergei A; Soldati, Thierry

doi:10.1111/j.1600-0854.2011.01296.x

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Title		Dynamin A, Myosin IB and Abp1 couple phagosome maturation to F-actin binding
Authors		Gopaldass, Navin Andréw Patel, Devang Kratzke, Ramona Dieckmann, Régis Hausherr, Stéphanie Hagedorn, Monica Monroy, Roger Krüger, Julia Neuhaus, Eva M Hoffmann, Eik Hille, Katja Kuznetsov, Sergei A Soldati, Thierry show all authors [1 - 13]
Published in		Traffic. 2012, vol. 13, no. 1, p. 120-30
Abstract		The role of actin, class I myosins and dynamin in endocytic uptake processes is well characterized, but their role during endo-phagosomal membrane trafficking and maturation is less clear. In Dictyostelium, knockout of myosin IB (myoB) leads to a defect in membrane protein recycling from endosomes back to the plasma membrane. Here, we show that actin plays a central role in the morphology and function of the endocytic pathway. Indeed, latrunculin B (LatB) induces endosome tubulation, a phenotype also observed in dynamin A (dymA)-null cells. Knockout of dymA impairs phagosome acidification, whereas knockout of myoB delays reneutralization, a phenotype mimicked by a low dose of LatB. As a read out for actin-dependent processes during maturation, we monitored the capacity of purified phagosomes to bind F-actin in vitro, and correlated this with the presence of actin-binding and membrane-trafficking proteins. Phagosomes isolated from myoB-null cells showed an increased binding to F-actin, especially late phagosomes. In contrast, early phagosomes from dymA-null cells showed reduced binding to F-actin while late phagosomes were unaffected. We provide evidence that Abp1 is the main F-actin-binding protein in this assay and is central for the interplay between DymA and MyoB during phagosome maturation.
Identifiers		DOI: 10.1111/j.1600-0854.2011.01296.x PMID: 22008230
Full text		Article (Published version) (1.2 MB) - Free access
Structures		Faculté des sciences / Section de chimie et biochimie / Département de biochimie
Citation (ISO format)		GOPALDASS, Navin Andréw et al. Dynamin A, Myosin IB and Abp1 couple phagosome maturation to F-actin binding. In: Traffic, 2012, vol. 13, n° 1, p. 120-30. doi: 10.1111/j.1600-0854.2011.01296.x https://archive-ouverte.unige.ch/unige:18866