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Applications of a post-column fluorigenic reaction in liquid chromatography for the determination of glucose and fructose in biological matrices

Publié dansAnalytica chimica acta, vol. 252, no. 1-2, p. 173-179
Date de publication1991
Résumé

A post-column fluorigenic reaction with benzamidine coupled to liquid chromatographic separation was used for the determination of reducing carbohydrates in three complex biological samples. This method allows the simultaneous determination of the different reducing sugars, which represents an improvement over batch enzymatic tests where in most instances only single monosaccharides can be determined. This selective liquid chromatographic method has a broad linear range. The method was validated by simultaneous analyses with a specific enzymatic test for glucose. Results are presented for the determination of glucose in human serum and mustard plants and glucose and fructose in white wines.

Mots-clés
  • Fluorimetry
  • Liquid chromatography
  • Biological samples
  • Carbohydrates
  • Fructose
  • Glucose
  • Plant materials
  • Serum
  • Wines
Citation (format ISO)
COQUET, Agnes Marianne Luce et al. Applications of a post-column fluorigenic reaction in liquid chromatography for the determination of glucose and fructose in biological matrices. In: Analytica chimica acta, 1991, vol. 252, n° 1-2, p. 173–179. doi: 10.1016/0003-2670(91)87212-P
Identifiants
ISSN du journal0003-2670
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Création13/10/2011 11:43:00
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