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Applications of a post-column fluorigenic reaction in liquid chromatography for the determination of glucose and fructose in biological matrices

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Published in Analytica Chimica Acta. 1991, vol. 252, no. 1-2, p. 173-179
Abstract A post-column fluorigenic reaction with benzamidine coupled to liquid chromatographic separation was used for the determination of reducing carbohydrates in three complex biological samples. This method allows the simultaneous determination of the different reducing sugars, which represents an improvement over batch enzymatic tests where in most instances only single monosaccharides can be determined. This selective liquid chromatographic method has a broad linear range. The method was validated by simultaneous analyses with a specific enzymatic test for glucose. Results are presented for the determination of glucose in human serum and mustard plants and glucose and fructose in white wines.
Keywords FluorimetryLiquid chromatographyBiological samplesCarbohydratesFructoseGlucosePlant materialsSerumWines
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COQUET, Agnes Marianne Luce et al. Applications of a post-column fluorigenic reaction in liquid chromatography for the determination of glucose and fructose in biological matrices. In: Analytica Chimica Acta, 1991, vol. 252, n° 1-2, p. 173-179. https://archive-ouverte.unige.ch/unige:17927

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