Scientific article
Open access

Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus

Published inBiochimica et biophysica acta. Bioenergetics, vol. 1837, no. 9, no. Photosynthesis Research for Sustainability: Keys to Produce Clean Energy, p. 1548-1552
Publication date2014-09
First online date2013-12-12

A repressible/inducible chloroplast gene expression system has been used to conditionally inhibit chloroplast protein synthesis in the unicellular alga Chlamydomonas reinhardtii. This system allows one to follow the fate of photosystem II and photosystem I and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII, the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the biosynthesis and turnover of individual photosynthetic complexes in the thylakoid membranes.

  • Chlamydomonas
  • Chloroplast
  • Gene expression
  • Photosystem degradation
  • European Commission - Thylakoid membrane in action: acclimation strategies in algae and plants [281341]
Citation (ISO format)
DINC, Emine et al. Repressible chloroplast gene expression in <i>Chlamydomonas</i>: A new tool for the study of the photosynthetic apparatus. In: Biochimica et biophysica acta. Bioenergetics, 2014, vol. 1837, n° 9, p. 1548–1552. doi: 10.1016/j.bbabio.2013.11.020
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ISSN of the journal0005-2728

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