Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus

Article Article scientifique Article Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus eng 2013-12-12 2014-09 author Dinc Emine 769321 author Ramundo Silvia 648848 author Croce Roberta author Rochaix Jean-David 25289 Administration de la faculté des sciences (SIAD) 1548-1552 10.1016/j.bbabio.2013.11.020 24333785 0005-2728 https://linkinghub.elsevier.com/retrieve/pii/S0005272813002156 A repressible/inducible <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/chloroplast-gene" rel="noopener noreferrer" target="_blank">chloroplast gene</a> expression system has been used to conditionally inhibit chloroplast <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/protein-biosynthesis" rel="noopener noreferrer" target="_blank">protein synthesis</a> in the unicellular alga <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/chlamydomonas-reinhardtii" rel="noopener noreferrer" target="_blank">Chlamydomonas reinhardtii</a>. This system allows one to follow the fate of <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/photosystem-ii" rel="noopener noreferrer" target="_blank">photosystem II</a> and <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/photosystem-i" rel="noopener noreferrer" target="_blank">photosystem I</a> and their antennae upon cessation of chloroplast translation. The main results are that the levels of the PSI core proteins decrease at a slower rate than those of PSII. Amongst the light-harvesting complexes, the decrease of CP26 proceeds at the same rate as for the PSII core proteins whereas it is significantly slower for CP29, and for the antenna complexes of PSI this rate is comprised between that of CP26 and CP29. In marked contrast, the components of trimeric LHCII, the major PSII antenna, persist for several days upon inhibition of chloroplast translation. This system offers new possibilities for investigating the <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/anabolism" rel="noopener noreferrer" target="_blank">biosynthesis</a> and turnover of individual photosynthetic complexes in the <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/thylakoid-membrane" rel="noopener noreferrer" target="_blank">thylakoid membranes</a>. Chlamydomonas Chloroplast Gene expression Photosystem degradation Dewey 580 European Commission Thylakoid membrane in action: acclimation strategies in algae and plants 281341 ASAP EU FP7 45 Département des sciences végétales 163 false Biochimica et biophysica acta. Bioenergetics 1837 9 Photosynthesis Research for Sustainability: Keys to Produce Clean Energy ca5bda04-c3d5-479f-82d4-bf8c42fb3bd8 Article (Published version) Public ROCHAIX01.pdf 500989 application/pdf CC BY e20cb86ddf280cbde888a4fb7bee24b7f18983b4674c23d9340ebefbe59b3bfc openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Repressible chloroplast gene expression in Chlamydomonas: A new tool for the study of the photosynthetic apparatus	Biochimica et biophysica acta. Bioenergetics			2014	51	137

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