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Scientific article
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Enrichment of complexing analytes on a copper-loaded silica surface and on-line coupling with high-performance liquid chromatography, using L-tryptophan and L-tyrosine as model compounds

Published inJournal of chromatography, vol. 393, no. 1, p. 51-56
Publication date1987-04
Abstract

A chemically modified silica (bisdithiocarbamate) strongly binds copper and has a loading capacity of 0.5 mmol g-1 for the copper ion. This new metal-loaded silica, packed in a short stainless-steel pre-column, is highly selective for the enrichment of two amino acids chosen as model compounds. On-line coupling of this precolumn with an analytical RP-18 column allowed the separation and detection of l-tryptophan and l-tyrosine at parts per billion levels in aqueous media with good accuracy. Separation was achieved by ion-pair chromatography and the solutes were detected by fluorescence. The ions generally present in natural aqueous media did not interfere.

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Citation (ISO format)
VEUTHEY, Jean-Luc, BAGNOUD, Marc André, HAERDI, Werner. Enrichment of complexing analytes on a copper-loaded silica surface and on-line coupling with high-performance liquid chromatography, using <sub>L</sub>-tryptophan and <sub>L</sub>-tyrosine as model compounds. In: Journal of chromatography, 1987, vol. 393, n° 1, p. 51–56. doi: 10.1016/S0021-9673(01)94203-7
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ISSN of the journal0021-9673
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