en
Scientific article
Open access
English

YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries

Published inNucleic acids research, vol. 47, no. D1, p. D1245-D1249
Publication date2019-01-08
First online date2018-10-24
Abstract

The ability to measure the abundance and visualize the localization of proteins across the yeast proteome has stimulated hypotheses on gene function and fueled discoveries. While the classic C’ tagged GFP yeast library has been the only resource for over a decade, the recent development of the SWAT technology has led to the creation of multiple novel yeast libraries where new-generation fluorescent reporters are fused at the N’ and C’ of open reading frames. Efficient access to these data requires a user interface to visualize and compare protein abundance, localization and co-localization across cells, strains, and libraries. YeastRGB (www.yeastRGB.org) was designed to address such a need, through a user-friendly interface that maximizes informative content. It employs a compact display where cells are cropped and tiled together into a ‘cell-grid.’ This representation enables viewing dozens of cells for a particular strain within a display unit, and up to 30 display units can be arrayed on a standard high-definition screen. Additionally, the display unit allows users to control zoom-level and overlay of images acquired using different color channels. Thus, YeastRGB makes comparing abundance and localization efficient, across thousands of cells from different strains and libraries.

eng
Affiliation Not a UNIGE publication
Funding
Citation (ISO format)
DUBREUIL, Benjamin et al. YeastRGB: comparing the abundance and localization of yeast proteins across cells and libraries. In: Nucleic acids research, 2019, vol. 47, n° D1, p. D1245–D1249. doi: 10.1093/nar/gky941
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ISSN of the journal0305-1048
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