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Scientific article
English

Genome-wide SWAp-Tag yeast libraries for proteome exploration

Published inNature methods, vol. 15, no. 8, p. 617-622
Publication date2018-07-09
First online date2018-07-09
Abstract

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

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Affiliation Not a UNIGE publication
Funding
  • European Commission - Systematic exploration of peroxisomal structure and function [646604]
Citation (ISO format)
WEILL, Uri et al. Genome-wide SWAp-Tag yeast libraries for proteome exploration. In: Nature methods, 2018, vol. 15, n° 8, p. 617–622. doi: 10.1038/s41592-018-0044-9
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ISSN of the journal1548-7091
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