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Scientific article
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English

PDB-wide identification of physiological hetero-oligomeric assemblies based on conserved quaternary structure geometry

Published inStructure, vol. 29, no. 11, p. 1303-1311.e3
Publication date2021-11
Abstract

An accurate understanding of biomolecular mechanisms and diseases requires information on protein quaternary structure (QS). A critical challenge in inferring QS information from crystallography data is distinguishing biological interfaces from fortuitous crystal-packing contacts. Here, we employ QS conservation across homologs to infer the biological relevance of hetero-oligomers. We compare the structures and compositions of hetero-oligomers, which allow us to annotate 7,810 complexes as physiologically relevant, 1,060 as likely errors, and 1,432 with comparative information on subunit stoichiometry and composition. Excluding immunoglobulins, these annotations encompass over 51% of hetero-oligomers in the PDB. We curate a dataset of 577 hetero-oligomeric complexes to benchmark these annotations, which reveals an accuracy >94%. When homology information is not available, we compare QS across repositories (PDB, PISA, and EPPIC) to derive confidence estimates. This work provides high-quality annotations along with a large benchmark dataset of hetero-assemblies.

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Keywords
  • Protein Data Bank
  • Crystal interfaces
  • Hetero-oligomers
  • Physiological assembly
  • Protein complexes benchmark
  • Protein interactions
  • Protein quaternary structure
  • Protein structure
  • Structural similarity
  • Structure prediction
Affiliation Not a UNIGE publication
Citation (ISO format)
DEY, Sucharita, LEVY, Emmanuel. PDB-wide identification of physiological hetero-oligomeric assemblies based on conserved quaternary structure geometry. In: Structure, 2021, vol. 29, n° 11, p. 1303–1311.e3. doi: 10.1016/j.str.2021.07.012
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ISSN of the journal0969-2126
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