en
Scientific article
Review
Open access
English

QSalignWeb: A Server to Predict and Analyze Protein Quaternary Structure

Published inFrontiers in molecular biosciences, vol. 8, 787510
Publication date2022-01-05
First online date2022-01-05
Abstract

The identification of physiologically relevant quaternary structures (QSs) in crystal lattices is challenging. To predict the physiological relevance of a particular QS, QSalign searches for homologous structures in which subunits interact in the same geometry. This approach proved accurate but was limited to structures already present in the Protein Data Bank (PDB). Here, we introduce a webserver ( www.QSalign.org ) allowing users to submit homo-oligomeric structures of their choice to the QSalign pipeline. Given a user-uploaded structure, the sequence is extracted and used to search homologs based on sequence similarity and PFAM domain architecture. If structural conservation is detected between a homolog and the user-uploaded QS, physiological relevance is inferred. The web server also generates alternative QSs with PISA and processes them the same way as the query submitted to widen the predictions. The result page also shows representative QSs in the protein family of the query, which is informative if no QS conservation was detected or if the protein appears monomeric. These representative QSs can also serve as a starting point for homology modeling.

eng
Keywords
  • Crystal contact
  • Physiological interface
  • Protein evolution
  • Protein interactions
  • Protein quaternary structure
  • Protein structure alignment
  • Protein superposition
  • Web server
Affiliation Not a UNIGE publication
Citation (ISO format)
DEY, Sucharita, PRILUSKY, Jaime, LEVY, Emmanuel. QSalignWeb: A Server to Predict and Analyze Protein Quaternary Structure. In: Frontiers in molecular biosciences, 2022, vol. 8, p. 787510. doi: 10.3389/fmolb.2021.787510
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal2296-889X
18views
3downloads

Technical informations

Creation04/26/2024 12:51:54 PM
First validation05/07/2024 12:40:38 PM
Update time05/07/2024 12:40:38 PM
Status update05/07/2024 12:40:38 PM
Last indexation05/07/2024 12:40:59 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack