en
Scientific article
Review
Open access
English

Considerations when investigating lncrna function in vivo

Published ineLife, vol. 3, p. 1-14; e03058
Publication date2014-08-14
First online date2014-08-14
Abstract

Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the lncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of lncRNAs in the light of different models of lncRNA molecular mechanism, and reflect on the design of experiments to mutate lncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.

eng
Keywords
  • Science forum
  • Brain development
  • Developmental defect
  • Knockout mouse models
  • Lethality
  • Long non-coding RNAs
Funding
  • Biotechnology and Biological Sciences Research Council - [BBS/E/T/000PR5885]
  • Medical Research Council - [G1000801B]
  • European Research Council - [SystemsHOX.ch]
  • National Human Genome Research Institute - [U54 HG007004-2]
  • Max-Planck-Gesellschaft -
  • Swiss National Science Foundation -
  • Cancer Research UK - [11832]
  • NCI NIH HHS - [P30 CA045508]
  • NHGRI NIH HHS - [U54 HG007004]
  • Austrian Academy of Sciences -
  • Austrian Science Fund FWF - [FWF F4302-B09]
  • European Molecular Biology Laboratory -
  • Wellcome Trust - Core funding renewal for the Wellcome Trust Centre for Cell Biology . [092076]
Citation (ISO format)
BASSETT, Andrew R. et al. Considerations when investigating lncrna function in vivo. In: eLife, 2014, vol. 3, p. 1–14. doi: 10.7554/elife.03058
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Article (Published version)
Identifiers
ISSN of the journal2050-084X
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