en
Scientific article
Open access
English

High-resolution mapping of mitotic DNA synthesis under conditions of replication stress in cultured cells

Published inSTAR protocols, vol. 4, no. 1, 101970
Publication date2023-03
Abstract

Cells experiencing DNA replication stress enter mitosis with under-replicated DNA, which activates a repair mechanism known as mitotic DNA synthesis (MiDAS). Here we describe a protocol to identify at genome wide and at high resolution the genomic sites where MiDAS occurs in cells exposed to aphidicolin. We use EdU incorporation to label nascent DNA in mitotic cells, followed by isolation of the EdU-labeled DNA and next-generation sequencing.

eng
Keywords
  • Cell Biology
  • Cell culture
  • ChIPseq
  • Molecular Biology
  • Sequencing
Funding
Citation (ISO format)
GROELLY, Florian J. et al. High-resolution mapping of mitotic DNA synthesis under conditions of replication stress in cultured cells. In: STAR protocols, 2023, vol. 4, n° 1, p. 101970. doi: 10.1016/j.xpro.2022.101970
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal2666-1667
12views
20downloads

Technical informations

Creation04/12/2024 11:58:39 AM
First validation04/24/2024 1:17:49 PM
Update time04/24/2024 1:17:49 PM
Status update04/24/2024 1:17:49 PM
Last indexation04/24/2024 1:18:10 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack