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Scientific article
Open access
English

Time‐sequenced transcriptomes of developing distal mouse limb buds: A comparative tissue layer analysis

Published inDevelopmental dynamics, vol. 251, no. 9, p. 1550-1575
Publication date2021-07-17
First online date2021-07-17
Abstract

Background

The development of the amniote limb has been an important model system to study patterning mechanisms and morphogenesis. For proper growth and patterning, it requires the interaction between the distal sub‐apical mesenchyme and the apical ectodermal ridge (AER) that involve the separate implementation of coordinated and tissue‐specific genetic programs.

Results

Here, we produce and analyze the transcriptomes of both distal limb mesenchymal progenitors and the overlying ectodermal cells, following time‐coursed dissections that cover from limb bud initiation to fully patterned limbs. The comparison of transcriptomes within each layer as well as between layers over time, allowed the identification of specific transcriptional signatures for each of the developmental stages. Special attention was given to the identification of genes whose transcription dynamics suggest a previously unnoticed role in the context of limb development and also to signaling pathways enriched between layers.

Conclusion

We interpret the transcriptomic data in light of the known development pattern and we conclude that a major transcriptional transition occurs in distal limb buds between E9.5 and E10.5, coincident with the switch from an early phase continuation of the signature of trunk progenitors, related to the initial proximo distal specification, to a late intrinsic phase of development.

eng
Keywords
  • AER
  • Hox genes
  • RNA-seq
  • Limb patterning
  • Limb progenitors
  • Signaling
  • Transcriptome
Citation (ISO format)
FERNANDEZ‐GUERRERO, Marc et al. Time‐sequenced transcriptomes of developing distal mouse limb buds: A comparative tissue layer analysis. In: Developmental dynamics, 2021, vol. 251, n° 9, p. 1550–1575. doi: 10.1002/dvdy.394
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ISSN of the journal1058-8388
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