Scientific article
Open access

Developmental and evolutionary comparative analysis of a regulatory landscape in mouse and chicken

Published inDevelopment, vol. 149, no. 12, dev200594
Publication date2022-06-15
First online date2022-06-30

Modifications in gene regulation are driving forces in the evolution of organisms. Part of these changes involve cis-regulatory elements (CREs), which contact their target genes through higher-order chromatin structures. However, how such architectures and variations in CREs contribute to transcriptional evolvability remains elusive. We use Hoxd genes as a paradigm for the emergence of regulatory innovations, as many relevant enhancers are located in a regulatory landscape highly conserved in amniotes. Here, we analysed their regulation in murine vibrissae and chicken feather primordia, two skin appendages expressing different Hoxd gene subsets, and compared the regulation of these genes in these appendages with that in the elongation of the posterior trunk. In the two former structures, distinct subsets of Hoxd genes are contacted by different lineage-specific enhancers, probably as a result of using an ancestral chromatin topology as an evolutionary playground, whereas the gene regulation that occurs in the mouse and chicken embryonic trunk partially relies on conserved CREs. A high proportion of these non-coding sequences active in the trunk have functionally diverged between species, suggesting that transcriptional robustness is maintained, despite considerable divergence in enhancer sequences.

  • Chromatin topology
  • Development
  • Enhancers
  • Evolution
  • Gene regulation
  • Placodes
  • TADs
  • Teguments
Citation (ISO format)
HINTERMANN, Aurélie et al. Developmental and evolutionary comparative analysis of a regulatory landscape in mouse and chicken. In: Development, 2022, vol. 149, n° 12, p. dev200594. doi: 10.1242/dev.200594
Main files (1)
Article (Published version)
ISSN of the journal0950-1991

Technical informations

Creation03/24/2024 9:23:12 AM
First validation04/22/2024 9:39:47 AM
Update time04/22/2024 9:39:47 AM
Status update04/22/2024 9:39:47 AM
Last indexation05/06/2024 6:26:05 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack