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Scientific article
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PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos

Published inDevelopment, vol. 145, dev.164988
Errata
Publication date2018-01-01
First online date2018-01-01
Abstract

Asymmetric division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. Here, we discovered that the plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) localizes to polarized dynamic structures in C. elegans zygotes, distributing in a PAR-dependent manner along the anterior-posterior (A-P) embryonic axis. PIP2 cortical structures overlap with F-actin, and coincide with the actin regulators RHO-1, CDC-42 as well as ECT-2. Particle image velocimetry analysis revealed that PIP2 and F-actin cortical movements are coupled, with PIP2 structures moving slightly ahead. Importantly, we established that PIP2 cortical structure formation and movement is actin-dependent. Conversely, we found that decreasing or increasing the level of PIP2 results in severe F-actin disorganization, revealing interdependence between these components. Furthermore, we uncovered that PIP2 and F-actin regulate the sizing of PAR cortical domains, including during the maintenance phase of polarization. Overall, our work establishes that a lipid membrane component, PIP2, modulates actin organization and cell polarity in C. elegans embryos.

eng
Keywords
  • Actin
  • Asymmetric cell division
  • C. elegans embryo
  • PAR polarity
  • PIP2
  • Phosphoinositides
Affiliation Not a UNIGE publication
Citation (ISO format)
SCHOLZE, Melina et al. PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in <i>Caenorhabditis elegans </i>embryos. In: Development, 2018, vol. 145, p. dev.164988. doi: 10.1242/dev.164988
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ISSN of the journal0950-1991
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