PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos

Article Article scientifique Article PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos eng 2018-01-01 2018-01-01 author Scholze Melina 0000-0003-1342-7858 Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland author Barbieux Kévin S. 0000-0003-4374-8484 Geodetic Engineering Laboratory (TOPO), Swiss Federal Institute of Technology (EPFL), Environmental Engineering Institute (IIE), Lausanne Switzerland author De Simone Alessandro Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland 985726 Département de génétique et évolution (Genev) (BIANI) author Boumasmoud Mathilde Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland author Suess Camille Cécile Nelly Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland 806934 Section des sciences pharmaceutiques (FARMA) author Wang Ruijia Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland author Gonczy Pierre Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland 742409 dev.164988 10.1242/dev.164988 29724757 0950-1991 https://journals.biologists.com/dev/article/doi/10.1242/dev.164988/264759/PI-4-5-P2-forms-dynamic-cortical-structures-and Asymmetric division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. Here, we discovered that the plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) localizes to polarized dynamic structures in C. elegans zygotes, distributing in a PAR-dependent manner along the anterior-posterior (A-P) embryonic axis. PIP2 cortical structures overlap with F-actin, and coincide with the actin regulators RHO-1, CDC-42 as well as ECT-2. Particle image velocimetry analysis revealed that PIP2 and F-actin cortical movements are coupled, with PIP2 structures moving slightly ahead. Importantly, we established that PIP2 cortical structure formation and movement is actin-dependent. Conversely, we found that decreasing or increasing the level of PIP2 results in severe F-actin disorganization, revealing interdependence between these components. Furthermore, we uncovered that PIP2 and F-actin regulate the sizing of PAR cortical domains, including during the maintenance phase of polarization. Overall, our work establishes that a lipid membrane component, PIP2, modulates actin organization and cell polarity in C. elegans embryos. Actin Asymmetric cell division C. elegans embryo PAR polarity PIP2 Phosphoinositides true Development 145 a7c87707-7e77-4b4b-a06c-eb146adbb70b Article (Published version) Restricted Article-scientifique-from-unpaywall.pdf 13673024 application/pdf eb293b2644f4e339ec1618eab9ccd6f7635ba8ed39fcc9fa65de786b284e1103 00e1a4b4-1c24-40df-99b4-286eaed61a96 Corrigendum Restricted dev169144.pdf 86032 application/pdf 002ce6b87ca1684ba0aa24fa72de23efbc5711ef17ee62ab025660f80a943493 Correction 10.1242/dev.169144 restrictedAccess

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PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos	Development			2018	54	0

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