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Scientific article
Open access
English

Protocol for the rapid intravenous in ovo injection of developing amniote embryos

Published inSTAR protocols, vol. 4, no. 2, p. 1-10; 102324
Publication date2023-06-16
Abstract

We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise intravenous injection. In addition to chicken embryos, this protocol is applicable to other amniote species that lay hard-shell eggs, including crocodiles and tortoises. This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists.

eng
Keywords
  • Developmental Biology
  • Evolutionary Biology
  • Model Organisms
Citation (ISO format)
COOPER, Rory, SANTOS DURAN, Gabriel, MILINKOVITCH, Michel C. Protocol for the rapid intravenous <i>in ovo</i> injection of developing amniote embryos. In: STAR protocols, 2023, vol. 4, n° 2, p. 1–10. doi: 10.1016/j.xpro.2023.102324
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ISSN of the journal2666-1667
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Technical informations

Creation04/13/2024 7:46:22 AM
First validation04/22/2024 8:00:48 AM
Update time04/22/2024 8:00:48 AM
Status update04/22/2024 8:00:48 AM
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