Protocol for the rapid intravenous in ovo injection of developing amniote embryos

Article Article scientifique Article Protocol for the rapid intravenous in ovo injection of developing amniote embryos eng 2023-06-16 author Cooper Rory 932989 Département de génétique et évolution (Genev) (BIANI) author Santos Duran Gabriel 854741 author Milinkovitch Michel C. 0000-0002-2553-0724 679786 Département de génétique et évolution (Genev) (BIANI) 1-10 102324 10.1016/j.xpro.2023.102324 37210721 2666-1667 PMC10209871 https://linkinghub.elsevier.com/retrieve/pii/S2666166723002915 We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise intravenous injection. In addition to chicken embryos, this protocol is applicable to other amniote species that lay hard-shell eggs, including crocodiles and tortoises. This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists. Developmental Biology Evolutionary Biology Model Organisms Dewey 590 Swiss National Science Foundation Evaluating the roles of geometry and physical processes in the development and evolution of skin appendages and skin colour patterns in reptiles 179431 CH Projects 44 Département de génétique et évolution 162 false STAR protocols 4 2 c57dccfa-d487-4196-b917-7c05ff745609 Article (Published version) Public MILINKOVITCH01.pdf 4166929 application/pdf CC BY-4.0 f953735c58560c909c94d1cc94720c4919d01e31d6d0617eca2a3ec7ba92aac7 openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Protocol for the rapid intravenous in ovo injection of developing amniote embryos	STAR protocols			2023	76	100

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Santos Duran, Gabriel