en
Scientific article
Open access
English

Islets-on-chip : a tool for real-time assessment of islet function prior to transplantation

Published inTransplant international, vol. 36, 11512
Publication date2023
First online date2023-10-11
Abstract

Islet transplantation improves metabolic control in patients with unstable type 1 diabetes. Clinical outcomes have been improving over the last decade, and the widely used beta-score allows the evaluation of transplantation results. However, predictive pre-transplantation criteria of islet quality for clinical outcomes are lacking. In this proof-of-concept study, we examined whether characterization of the electrical activity of donor islets could provide a criterion. Aliquots of 8 human donor islets from the STABILOT study, sampled from islet preparations before transplantation, were characterized for purity and split for glucose-induced insulin secretion and electrical activity using multi-electrode-arrays. The latter tests glucose concentration dependencies, biphasic activity, hormones, and drug effects (adrenalin, GLP-1, glibenclamide) and provides a ranking of CHIP-scores from 1 to 6 (best) based on electrical islet activity. The analysis was performed online in real time using a dedicated board or offline. Grouping of beta-scores and CHIP-scores with high, intermediate, and low values was observed. Further analysis indicated correlation between CHIP-score and beta-score, although significance was not attained (R = 0.51,p= 0.1). This novel approach is easily implantable in islet isolation units and might provide means for the prediction of clinical outcomes. We acknowledge the small cohort size as the limitation of this pilot study.

eng
Keywords
  • Diabetes mellitus
  • Electrophysiology
  • Islet
  • Islet transplantation
  • Multielectrode array
  • Transplant assessment
  • Humans
  • Islets of Langerhans
  • Insulin / metabolism
  • Blood Glucose / analysis
  • Pilot Projects
  • Islets of Langerhans Transplantation / methods
  • Diabetes Mellitus, Type 1 / surgery
  • Glucose / metabolism
  • Glucose / pharmacology
Citation (ISO format)
RAOUX, Matthieu et al. Islets-on-chip : a tool for real-time assessment of islet function prior to transplantation. In: Transplant international, 2023, vol. 36, p. 11512. doi: 10.3389/ti.2023.11512
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Article (Published version)
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ISSN of the journal0934-0874
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Creation01.11.2023 14:26:21
First validation27.03.2024 13:46:22
Update time27.03.2024 13:46:22
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