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Title

Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo

Authors
Legrain, P
Bilbe, G
Published in Nucleic Acids Research. 1994, vol. 22, no. 24, p. 5223-8
Abstract A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution.
Keywords Amino Acid SequenceBase SequenceCloning, MolecularDNA, Complementary/geneticsEscherichia coli/geneticsFungal Proteins/geneticsGenes/geneticsGenes, Fungal/geneticsHela CellsHumansMolecular Sequence DataMolecular WeightMutation/physiologyPhenotypeRNA-Binding Proteins/chemistry/genetics/isolation & purificationRecombinant Fusion Proteins/biosynthesisRibonucleoprotein, U2 Small NuclearSaccharomyces cerevisiae/chemistrySaccharomyces cerevisiae ProteinsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidZinc Fingers/genetics/physiology
Identifiers
PMID: 7816610
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KRAEMER, Angela et al. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. In: Nucleic Acids Research, 1994, vol. 22, n° 24, p. 5223-8. https://archive-ouverte.unige.ch/unige:17580

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Deposited on : 2011-11-28

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