

Other version: http://preview.ncbi.nlm.nih.gov/pubmed/7816610
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Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo |
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Published in | Nucleic acids research. 1994, vol. 22, no. 24, p. 5223-8 | |
Abstract | A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution. | |
Keywords | Amino Acid Sequence — Base Sequence — Cloning, Molecular — DNA, Complementary/genetics — Escherichia coli/genetics — Fungal Proteins/genetics — Genes/genetics — Genes, Fungal/genetics — Hela Cells — Humans — Molecular Sequence Data — Molecular Weight — Mutation/physiology — Phenotype — RNA-Binding Proteins/chemistry/genetics/isolation & purification — Recombinant Fusion Proteins/biosynthesis — Ribonucleoprotein, U2 Small Nuclear — Saccharomyces cerevisiae/chemistry — Saccharomyces cerevisiae Proteins — Sequence Alignment — Sequence Analysis, DNA — Sequence Homology, Amino Acid — Zinc Fingers/genetics/physiology | |
Identifiers | PMID: 7816610 | |
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![]() ![]() Other version: http://preview.ncbi.nlm.nih.gov/pubmed/7816610 |
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Citation (ISO format) | KRAEMER, Angela et al. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. In: Nucleic acids research, 1994, vol. 22, n° 24, p. 5223-8. doi: 10.1093/nar/22.24.5223 https://archive-ouverte.unige.ch/unige:17580 |