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Scientific article
English

Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo

Published inNucleic acids research, vol. 22, no. 24, p. 5223-5228
Publication date1994
Abstract

A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution.

Keywords
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • Escherichia coli/genetics
  • Fungal Proteins/genetics
  • Genes/genetics
  • Genes, Fungal/genetics
  • Hela Cells
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Mutation/physiology
  • Phenotype
  • RNA-Binding Proteins/chemistry/genetics/isolation & purification
  • Recombinant Fusion Proteins/biosynthesis
  • Ribonucleoprotein, U2 Small Nuclear
  • Saccharomyces cerevisiae/chemistry
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Zinc Fingers/genetics/physiology
Citation (ISO format)
KRAEMER, Angela et al. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. In: Nucleic acids research, 1994, vol. 22, n° 24, p. 5223–5228. doi: 10.1093/nar/22.24.5223
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Article (Published version)
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Identifiers
ISSN of the journal0305-1048
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