Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo

Article Article scientifique Article Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo eng 1994 author Kraemer Angela 22808 author Legrain P author Mulhauser Frank 22591 author Groening Karsten Johannes 88149 author Brosi Reto 29189 author Bilbe G 5223-5228 10.1093/nar/22.24.5223 7816610 0305-1048 http://preview.ncbi.nlm.nih.gov/pubmed/7816610 A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chimeric protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution. Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary/genetics Escherichia coli/genetics Fungal Proteins/genetics Genes/genetics Genes, Fungal/genetics Hela Cells Humans Molecular Sequence Data Molecular Weight Mutation/physiology Phenotype RNA-Binding Proteins/chemistry/genetics/isolation & purification Recombinant Fusion Proteins/biosynthesis Ribonucleoprotein, U2 Small Nuclear Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Zinc Fingers/genetics/physiology Dewey 570 46 Département de biologie cellulaire 165 false Nucleic acids research 22 24 23f1728d-2fb0-4339-880e-efb68ad771f8 Article (Published version) Private fulltext.pdf 1358823 application/pdf closedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo	Nucleic acids research			1994	682	0

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