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Scientific article
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WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity

Published ineLife, vol. 9, e57205
Publication date2020-09-18
First online date2020-09-18
Abstract

Centrioles are characterized by a nine-fold arrangement of microtubule triplets held together by an inner protein scaffold. These structurally robust organelles experience strenuous cellular processes such as cell division or ciliary beating while performing their function. However, the molecular mechanisms underlying the stability of microtubule triplets, as well as centriole architectural integrity remain poorly understood. Here, using ultrastructure expansion microscopy for nanoscale protein mapping, we reveal that POC16 and its human homolog WDR90 are components of the microtubule wall along the central core region of the centriole. We further found that WDR90 is an evolutionary microtubule associated protein. Finally, we demonstrate that WDR90 depletion impairs the localization of inner scaffold components, leading to centriole structural abnormalities in human cells. Altogether, this work highlights that WDR90 is an evolutionary conserved molecular player participating in centriole architecture integrity.

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Keywords
  • Cell biology
  • Centriole
  • Chlamydomonas
  • Expansion microscopy
  • Human
  • Inner scaffold
  • Microtubule
Funding
Citation (ISO format)
STEIB, Emmanuelle et al. WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. In: eLife, 2020, vol. 9, p. e57205. doi: 10.7554/eLife.57205
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ISSN of the journal2050-084X
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