en
Scientific article
Open access
English

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)

Published inNature methods, vol. 19, no. 2, p. 216-222
Publication date2022-01-13
First online date2022-01-13
Abstract

Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.

eng
Funding
Citation (ISO format)
LAPORTE, Marine et al. Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). In: Nature methods, 2022, vol. 19, n° 2, p. 216–222. doi: 10.1038/s41592-021-01356-4
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal1548-7091
13views
4downloads

Technical informations

Creation02/15/2024 1:21:45 PM
First validation03/18/2024 10:22:09 AM
Update time03/18/2024 10:22:09 AM
Status update03/18/2024 10:22:09 AM
Last indexation05/06/2024 6:11:27 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack