en
Scientific article
Open access
English

The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing

Published inGenes & development, vol. 29, no. 6, p. 617-629
Publication date2015-03-15
First online date2015-03-11
Abstract

Piwi–piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions of most of the factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show that MOV10L1 exhibits 5′-to-3′ directional RNA-unwinding activity in vitro and that a point mutation that abolishes this activity causes a failure in primary piRNA biogenesis in vivo. We demonstrate that MOV10L1 selectively binds piRNA precursor transcripts and is essential for the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Multiple analyses suggest an intimate coupling of piRNA precursor processing with elements of local secondary structures such as G quadruplexes. Our results support a model in which MOV10L1 RNA helicase activity promotes unwinding and funneling of the single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing.

eng
Keywords
  • Argonaute
  • Armitage
  • G quadruplex
  • MOV10L1
  • Piwi
  • piRNA
Affiliation Not a UNIGE publication
Funding
  • NICHD NIH HHS - [HD069592]
  • National Institute of Health - [GM072777]
  • Nanjing Medical University - [2013RC02]
  • National Natural Science Foundation of China - [31471228]
  • National Research Science - [F31HD081892]
  • NIGMS NIH HHS - [R01 GM072777]
Citation (ISO format)
VOUREKAS, Anastassios et al. The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing. In: Genes & development, 2015, vol. 29, n° 6, p. 617–629. doi: 10.1101/gad.254631.114
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Article (Published version)
Identifiers
ISSN of the journal0890-9369
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