The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing

Article Article scientifique Article The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing eng 2015-03-11 2015-03-15 author Vourekas Anastassios author Zheng Ke author Fu Qi author Maragkakis Manolis 0000-0002-3158-1763 author Alexiou Panagiotis 0000-0003-3437-7482 author Ma Jing 920998 author Pillai Ramesh 854560 Département de biologie moléculaire et cellulaire (MOCEL) author Mourelatos Zissimos author Wang P. Jeremy 617-629 10.1101/gad.254631.114 25762440 0890-9369 PMC4378194 http://genesdev.cshlp.org/lookup/doi/10.1101/gad.254631.114 <p>Piwi–piRNA (Piwi-interacting RNA) ribonucleoproteins (piRNPs) enforce retrotransposon silencing, a function critical for preserving the genome integrity of germ cells. The molecular functions of most of the factors that have been genetically implicated in primary piRNA biogenesis are still elusive. Here we show that MOV10L1 exhibits 5′-to-3′ directional RNA-unwinding activity in vitro and that a point mutation that abolishes this activity causes a failure in primary piRNA biogenesis in vivo. We demonstrate that MOV10L1 selectively binds piRNA precursor transcripts and is essential for the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins. Multiple analyses suggest an intimate coupling of piRNA precursor processing with elements of local secondary structures such as G quadruplexes. Our results support a model in which MOV10L1 RNA helicase activity promotes unwinding and funneling of the single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing.</p> Argonaute Armitage G quadruplex MOV10L1 Piwi piRNA NICHD NIH HHS HD069592 National Institute of Health GM072777 Nanjing Medical University 2013RC02 National Natural Science Foundation of China 31471228 National Research Science F31HD081892 NIGMS NIH HHS R01 GM072777 true Genes & development 29 6 a298f50b-7814-4b19-a5cf-819b09c76f1d Article (Published version) Public Article-scientifique-from-unpaywall.pdf 1369675 application/pdf CC BY-NC-4.0 999b1fd9c26a5aa75b1a2900771adae95fbd6b7a8ac321cfa841c59ee3df5220 openAccess

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The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing	Genes & development			2015	108	215

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